 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 3 | 1/1 | 返回列表 |
| 查看: 480 | 回復(fù): 2 | ||
白玉浴血木蟲 (正式寫手)
|
[求助]
紫外可見吸收光譜，求大神幫忙解釋一下�。。�！
|
|
To study and quantify the oxidation of thiols within the multilayers, 1 μm silica particles coated with five bilayers of PMASH/PVPON were used. In reduced form, the multilayers are unstable at pH 7 and rapidly disassemble when placed into a 10 mM solution of Ellman’s reagent in 1 M phosphate buffer, pH 7.5. The resulting solution has a characteristic absorption at 412 nm (Figure 2, spectrum 1), which is used to quantify the number of thiol groups by using a calibration curve obtained with standard solutions of glutathione. The addition of CaT to the particles with PMASH/PVPON multilayers at different pH (4–6) results in a decreased number of thiol groups, reflecting their oxidation into disulfide linkages. We found that the addition of 2 mole equivalents of CaT oxidized 90% of thiols within 1 min (Figure 2, spectrum 2), and a moderate excess of CaT (2 mM solution) converts the thiols into disulfides nearly quantitatively (Figure 2, spectrum 3). 請(qǐng)大神指教，這里的曲線1是一條校準(zhǔn)曲線，這是校準(zhǔn)的什么，這條曲線代表什么意義呢？曲線2和3又是怎么得來的雙硫鍵的氧化率呢？ 圖片1.png |
回復(fù)此樓» 猜你喜歡
【求調(diào)劑】新能源材料本科，一志愿211，初試321
已經(jīng)有3人回復(fù)
-
材料考研調(diào)劑
已經(jīng)有3人回復(fù)
-
材料調(diào)劑
已經(jīng)有12人回復(fù)
-
英一數(shù)一408，總分284，二戰(zhàn)真誠求調(diào)劑
已經(jīng)有14人回復(fù)
-
085410 一志愿211 22408分?jǐn)?shù)359求調(diào)劑
已經(jīng)有4人回復(fù)
-
271求調(diào)劑
已經(jīng)有19人回復(fù)
-
385分 生物學(xué)（071000）求調(diào)劑
已經(jīng)有3人回復(fù)
-
一志愿安徽大學(xué)計(jì)算機(jī)科學(xué)與技術(shù)學(xué)碩，331分求調(diào)劑
已經(jīng)有3人回復(fù)
-
318求調(diào)劑，計(jì)算材料方向
已經(jīng)有8人回復(fù)
-
291求調(diào)劑
已經(jīng)有25人回復(fù)
mao931650079
新蟲 (小有名氣)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 300.9
- 散金: 33
- 紅花: 2
- 帖子: 75
- 在線: 32.8小時(shí)
- 蟲號(hào): 3486791
- 注冊(cè): 2014-10-20
- 性別: GG
- 專業(yè): 電化學(xué)分析
2樓2016-10-19 17:13:15
 |
3樓2016-10-19 18:35:58
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 白玉浴血 的主題更新
| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 材料調(diào)劑 +12 | 一樣YWY 2026-04-01 | 12/600 |
|
|---|---|---|---|---|
|
[考研] 385分 生物學(xué)（071000）求調(diào)劑 +3 | qf626 2026-04-01 | 3/150 |
|
|
|
[考研] 一志愿安徽大學(xué)計(jì)算機(jī)科學(xué)與技術(shù)學(xué)碩，331分求調(diào)劑 +3 | 蔣昌鵬qtj 2026-04-01 | 3/150 |
|
|
|
[考研] 江蘇科技大學(xué)招材料研究生 +4 | Su032713. 2026-04-01 | 5/250 |
|
|
|
[考研] 805600專碩材料與化工348分求調(diào)劑 +4 | 上學(xué)啦！ 2026-04-01 | 4/200 |
|
|
|
[考研] 316求調(diào)劑 +9 | 舟自梗 2026-04-01 | 10/500 |
|
|
|
[考研] 26考研調(diào)劑 +3 | Wnz.20030617 2026-04-01 | 3/150 |
|
|
|
[考研] 材料專碩306英一數(shù)二 +7 | z1z2z3879 2026-03-31 | 7/350 |
|
|
|
[考研] 336求調(diào)劑 +3 | kiyy 2026-04-01 | 3/150 |
|
|
|
[考研] 抱歉 +4 | 田洪有 2026-03-30 | 4/200 |
|
|
|
[考研]
 105500藥學(xué)求調(diào)劑，一志愿山東大學(xué)藥學(xué)，348分
+3
|
gr哈哈哈 2026-03-28 | 3/150 |
|
|
|
[考研] 求調(diào)劑 +10 | 家佳佳佳佳佳 2026-03-29 | 10/500 |
|
|
|
[考研] 342求調(diào)劑 +4 | 加油a李zs 2026-03-26 | 4/200 |
|
|
|
[考研] 085602 化學(xué)工程專碩 340分求調(diào)劑 +4 | qianbai11 2026-03-29 | 4/200 |
|
|
|
[考研] 296求調(diào)劑 +10 | 彼岸t 2026-03-29 | 10/500 |
|
|
|
[考研] 311求調(diào)劑 +10 | lin0039 2026-03-26 | 10/500 |
|
|
|
[考研] 085600，材料與化工321分求調(diào)劑 +10 | 大饞小子 2026-03-28 | 10/500 |
|
|
|
[考研] 279求調(diào)劑 +4 | 蝶舞輕繞 2026-03-29 | 4/200 |
|
|
|
[考研] 298求調(diào)劑 +4 | 種圣賜 2026-03-28 | 4/200 |
|
|
|
[考研] 11408軟件工程求調(diào)劑 +3 | Qiu學(xué)ing 2026-03-28 | 3/150 |
|