24小時(shí)熱門版塊排行榜

返回列表

zw0123456789zw

至尊木蟲 (職業(yè)作家)

Felix Baumgartner Space Jump

應(yīng)助: 278 (大學(xué)生)
金幣: 21212.9
散金: 286
紅花: 37
帖子: 3620
在線: 1696.7小時(shí)
蟲號(hào): 313787
注冊(cè): 2007-02-24
專業(yè): 天然藥物化學(xué)

[求助] Caco-2細(xì)胞與 HT29細(xì)胞區(qū)別已有1人參與

Caco-2細(xì)胞與 HT29細(xì)胞區(qū)別？有人知道嗎，求指教。

回復(fù)此樓

» 猜你喜歡

276求調(diào)劑。有半年電池和半年高分子實(shí)習(xí)經(jīng)歷已經(jīng)有10人回復(fù)
一志愿南航材料專317分求調(diào)劑已經(jīng)有4人回復(fù)
求調(diào)劑已經(jīng)有4人回復(fù)
生物學(xué)學(xué)碩求調(diào)劑已經(jīng)有5人回復(fù)
284求調(diào)劑已經(jīng)有10人回復(fù)
一志愿山東大學(xué)藥學(xué)學(xué)碩求調(diào)劑已經(jīng)有4人回復(fù)
07化學(xué)280分求調(diào)劑已經(jīng)有4人回復(fù)
298-一志愿中國農(nóng)業(yè)大學(xué)-求調(diào)劑已經(jīng)有12人回復(fù)
求材料，環(huán)境專業(yè)調(diào)劑已經(jīng)有3人回復(fù)
335求調(diào)劑已經(jīng)有5人回復(fù)

1樓 2017-02-22 21:28:42

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

samule1978

鐵桿木蟲 (正式寫手)

應(yīng)助: 125 (高中生)
金幣: 5461.5
紅花: 18
帖子: 782
在線: 214.3小時(shí)
蟲號(hào): 4139362
注冊(cè): 2015-10-13
性別: GG
專業(yè): 中藥質(zhì)量評(píng)價(jià)

【答案】應(yīng)助回帖

★ ★
感謝參與，應(yīng)助指數(shù) +1
zw0123456789zw: 金幣+2, ★有幫助, 謝謝 2017-02-23 18:17:24

HT29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies.

1964年，HT-29細(xì)胞系由J.Fogh 用移植培養(yǎng)方法和含15%FBS的F12培養(yǎng)液從原發(fā)性腫瘤分離。近來，已建株的培養(yǎng)細(xì)胞用含相同血清McCoy’s 5a培液培養(yǎng)。該細(xì)胞系在裸鼠中致瘤，也能在類固醇處理的地鼠中致瘤。這個(gè)細(xì)胞株合成IgA, CEA, 蛋白綁定TGFβ的分泌成分和粘液素。

組織來源: 結(jié)直腸腺癌
形態(tài): 上皮細(xì)胞

The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh.

The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation.

Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.

The Caco-2 cell line

*
Derived from a human colorectal carcinoma

*
With remarkable morphological and biochemical similarity to the small intestinal columnar epithelium

*
The apparent permeability coefficient (Papp) has been shown to correlate well with %HIA or %FA

*
Extremely useful for mechanistic studies of drug absorption and as an absorption screening assay for pre-clinical drug selection.

贊一下

回復(fù)此樓

2樓2017-02-23 14:25:57

已閱關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

相關(guān)版塊跳轉(zhuǎn) 我要訂閱樓主 natmed 的主題更新