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zw0123456789zw至尊木蟲 (職業(yè)作家)
Felix Baumgartner Space Jump
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[求助]
Caco-2細胞 與 HT29細胞 區(qū)別 已有1人參與
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| Caco-2細胞 與 HT29細胞 區(qū)別？ 有人知道嗎，求指教。 |
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samule1978
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【答案】應(yīng)助回帖
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感謝參與，應(yīng)助指數(shù) +1
zw0123456789zw: 金幣+2, ★有幫助, 謝謝 2017-02-23 18:17:24
感謝參與，應(yīng)助指數(shù) +1
zw0123456789zw: 金幣+2, ★有幫助, 謝謝 2017-02-23 18:17:24
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HT29 cells are human intestinal epithelial cells which produce the secretory component of Immunoglobulin A (IgA), and carcinoembryonic antigen (CEA). Cells are used for tumourigenicity studies. 1964年，HT-29細胞系由J.Fogh 用移植培養(yǎng)方法和含15%FBS的F12培養(yǎng)液從原發(fā)性腫瘤分離。近來，已建株的培養(yǎng)細胞用含相同血清McCoy’s 5a培液培養(yǎng)。 該細胞系在裸鼠中致瘤，也能在類固醇處理的地鼠中致瘤。 這個細胞株合成IgA, CEA, 蛋白綁定TGFβ的分泌成分和粘液素。 組織來源: 結(jié)直腸腺癌 形態(tài): 上皮細胞 The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation. Caco-2 may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery. The Caco-2 cell line * Derived from a human colorectal carcinoma * With remarkable morphological and biochemical similarity to the small intestinal columnar epithelium * The apparent permeability coefficient (Papp) has been shown to correlate well with %HIA or %FA * Extremely useful for mechanistic studies of drug absorption and as an absorption screening assay for pre-clinical drug selection. |
2樓2017-02-23 14:25:57
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