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油紅O染色步驟 已有2人參與
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請問有誰在脂肪細胞里面做個油紅O染色,急求步驟。 [ Last edited by 1949stone on 2009-11-21 at 21:32 ] |
金蟲 (正式寫手)
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Preparing Oil Red O Stain Prepare a stock solution by weighing out 300mg of Oil Red O powder and adding this to 100 mL of 99% isopropanol. This stock solution is stable one year from the date it was made. In the fume hood, mix 3 parts of Oil Red O stock solution with 2 parts DI water. Incubate 10 minutes at room temperature. This working solution is only stable for 2 hours. Place a piece of Whatman filter paper in a funnel above a vessel. Filter the Oil Red O working solution completely through the filter funnel. |
鐵蟲 (初入文壇)
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