| 3 | 1/1 | 返回列表 |
| 查看: 380 | 回復(fù): 2 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ,點(diǎn)擊這里進(jìn)行查看 | |||
[交流]
誰能幫我翻譯一下以下內(nèi)容?
|
|||
Cultures were allowed to grow at 30 ℃ with shaking at 200 rpm, in 250 ml conical flasks containing 50 ml aliquots pre-culture basal medium of the following composition (g/l) eptone, 20; Glucose, 30; KH2PO4, 1; MgSO4﹒7H2O, 0.5; and uric acid, 0.1. Then 0.5 ml of the overnight culture was centrifuged, washed with sterile saline and consequently used as inoculum for the basal production medium of the following composition (g/l): K3PO4, 10; MgCl2, 1; and uricacid, 3. Bacterial growth was monitored turbidimetrically at 550 nm. At the indicated time, 2 ml of the growing cultures was taken and centrifuged at 8000 rpm for 2 min.主要是這里的前后兩個(gè)培養(yǎng)基分別是什么用處,非常感謝! |
至尊木蟲 (正式寫手)
至尊木蟲 (正式寫手)
|
培養(yǎng)條件:在30 ℃,200 rpm,在250 ml錐形瓶中加入50ml經(jīng)過預(yù)處理的基礎(chǔ)培養(yǎng)基;A(chǔ)培養(yǎng)基成分如下:eptone20(估計(jì)是蛋白胨吧), ; Glucose, 30; KH2PO4, 1; MgSO4﹒7H2O, 0.5; and 尿酸, 0.1. 取過夜培養(yǎng)的基礎(chǔ)培養(yǎng)基0.5ml離心,沉淀用無菌生理鹽水洗滌后作為發(fā)酵種子。發(fā)酵培養(yǎng)基成分如下: (g/l): K3PO4, 10; MgCl2, 1; and uric acid, 3. 菌體生長(zhǎng)在550nm下檢測(cè)濁度.在指定時(shí)間,取2ml發(fā)酵液8000rpm離心2min |
| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿北京化工大學(xué)材料與化工 264分各科過A區(qū)國家線 +3 | 哈哈157349 2026-03-21 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 07化學(xué)280分求調(diào)劑 +4 | 722865 2026-03-23 | 4/200 |
|
|
[考研] 材料專碩找調(diào)劑 +3 | 哈哈哈吼吼吼哈 2026-03-23 | 3/150 |
|
|
[考研] 384求調(diào)劑 +3 | 子系博 2026-03-22 | 6/300 |
|
|
[考研] 化學(xué)308分求調(diào)劑 +3 | 你好明天你好 2026-03-23 | 3/150 |
|
|
[考研] 接收2026碩士調(diào)劑(學(xué)碩+專碩) +4 | allen-yin 2026-03-23 | 6/300 |
|
|
[考研] 求老師收我 +3 | zzh16938784 2026-03-23 | 3/150 |
|
|
[考研] 291求調(diào)劑 +5 | 孅華 2026-03-22 | 5/250 |
|
|
[考研] 276求調(diào)劑 +3 | YNRYG 2026-03-21 | 4/200 |
|
|
[考研] 307求調(diào)劑 +11 | 冷笙123 2026-03-17 | 11/550 |
|
|
[考研] 生物學(xué)071000 329分求調(diào)劑 +5 | 我愛生物生物愛?/a> 2026-03-17 | 5/250 |
|
|
[考研] 298求調(diào)劑一志愿211 +3 | 上岸6666@ 2026-03-20 | 3/150 |
|
|
[考博] 招收博士1-2人 +3 | QGZDSYS 2026-03-18 | 4/200 |
|
|
[考研] 材料學(xué)碩301分求調(diào)劑 +7 | Liyouyumairs 2026-03-21 | 7/350 |
|
|
[考研] 0703化學(xué)調(diào)劑 +4 | 妮妮ninicgb 2026-03-21 | 4/200 |
|
|
[考研] 299求調(diào)劑 +5 | shxchem 2026-03-20 | 7/350 |
|
|
[考研] 266求調(diào)劑 +3 | 哇呼哼呼哼 2026-03-20 | 3/150 |
|
|
[考研] 22 350 本科985求調(diào)劑,求老登收留 +3 | 李軼男003 2026-03-20 | 3/150 |
|
|
[考研] 0817 化學(xué)工程 299分求調(diào)劑 有科研經(jīng)歷 有二區(qū)文章 +22 | rare12345 2026-03-18 | 22/1100 |
|
|
[考博] 26博士申請(qǐng) +3 | 1042136743 2026-03-17 | 3/150 |
|