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[交流]
誰能幫我翻譯一下以下內(nèi)容?
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Cultures were allowed to grow at 30 ℃ with shaking at 200 rpm, in 250 ml conical flasks containing 50 ml aliquots pre-culture basal medium of the following composition (g/l) eptone, 20; Glucose, 30; KH2PO4, 1; MgSO4﹒7H2O, 0.5; and uric acid, 0.1. Then 0.5 ml of the overnight culture was centrifuged, washed with sterile saline and consequently used as inoculum for the basal production medium of the following composition (g/l): K3PO4, 10; MgCl2, 1; and uricacid, 3. Bacterial growth was monitored turbidimetrically at 550 nm. At the indicated time, 2 ml of the growing cultures was taken and centrifuged at 8000 rpm for 2 min.主要是這里的前后兩個(gè)培養(yǎng)基分別是什么用處,非常感謝! |
至尊木蟲 (正式寫手)
至尊木蟲 (正式寫手)
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培養(yǎng)條件:在30 ℃,200 rpm,在250 ml錐形瓶中加入50ml經(jīng)過預(yù)處理的基礎(chǔ)培養(yǎng)基;A(chǔ)培養(yǎng)基成分如下:eptone20(估計(jì)是蛋白胨吧), ; Glucose, 30; KH2PO4, 1; MgSO4﹒7H2O, 0.5; and 尿酸, 0.1. 取過夜培養(yǎng)的基礎(chǔ)培養(yǎng)基0.5ml離心,沉淀用無菌生理鹽水洗滌后作為發(fā)酵種子。發(fā)酵培養(yǎng)基成分如下: (g/l): K3PO4, 10; MgCl2, 1; and uric acid, 3. 菌體生長在550nm下檢測濁度.在指定時(shí)間,取2ml發(fā)酵液8000rpm離心2min |
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