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| 溫陽方由溫陽利水法化裁而來,是王老經長期臨床實踐總結的慢性心衰中藥復方制劑。前期臨床和實驗研究證實該方具有良好的臨床療效,能夠改善心力衰竭動物模型心肌重塑。隨著慢性心力衰竭研究進展的不斷深入,細胞因子網絡在心力衰竭心肌重構中的作用日益引起廣泛關注。其中腫瘤壞死因子α(Tumor necrosis factorα,TNFα) 是重要的促炎性細胞因子,作為激活細胞級聯(lián)反應的主要介質,在細胞因子網絡中發(fā)揮關鍵作用。核轉錄因子-κB (nuclear factor κB,NF-κB)是調控多種基因轉錄的關鍵因子,活化后能調控包括TNFα在內的多種炎癥介質的轉錄,作為炎性反應的整合者在免疫調節(jié)和炎癥發(fā)生過程中具有重要作用。為進一步探討溫陽方藥效和作用機理,明確NF-κB在心衰發(fā)病中的作用及對TNFα調節(jié)機制,本研究采用細胞培養(yǎng)和血清藥理學方法,開展溫陽方對腫瘤壞死因子α(TNFα)誘導新生大鼠心肌成纖維細胞(cardial fibroblasts CFs)增殖、膠原合成,及核轉錄因子NF-κB表達的影響,進一步從分子水平探討其抑制心肌纖維化,改善心力衰竭心肌重塑的作用機制,從而為其臨床應用及新藥開發(fā)提供科學理論和依據。 |
| By the WenYangFang WenYangLi water cut and come, is the long-term clinical practice cenwanglaoshan herbal preparations of chronic heart failure. The clinical and experimental research confirms the party has good curative effect, can improve cardiac remodeling animal model for heart failure. With chronic heart failure, research progress of the cytokine network in hf myocardial reconstruction is aroused widespread concern. Among Tumor necrosis factor alpha (Tumor necrosis, TNF alpha) in alpha is an important factor of inflammatory cells, promote cell activation cascade as the main medium, in cytokines play an important role in network. Transcription factor - κ B (nuclear κ B, NF - in κ B) is the regulation of gene transcription factors, activate the key to control including TNF alpha, inflammation, as the transcription of integration of the inflammatory response in immune adjustment and inflammation plays an important role in the process. For further discussion WenYangFang efficacy and mechanism, clear in heart failure κ B NF - the role and pathogenesis of TNF alpha adjusting mechanism, this study adopts cell culture and serum pharmacology, WenYangFang method of tumor necrosis factor (TNF alpha) inducing newborn rat cardiac fibroblasts cardial (CFs) proliferation, fibroblasts collagen synthesis, and κ transcription factor NF - expression of B, further discusses its suppression of the molecular myocardial fibrosis, improve cardiac remodeling of heart failure mechanism, so as to its clinical application and development of new medicine to provide scientific theory and evidence. |
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