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望月臺木蟲 (小有名氣)
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[交流]
【求助/交流】大腸桿菌BW25113基因型意思? 已有5人參與
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各位蟲友好: 大腸桿菌BW25113 網(wǎng)上查的genetype 是∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514,想請教一下,∆(araD-araB)567表示什么意思? 謝謝! PS:我知道BW25113不能降解阿拉伯糖,∆(araD-araB)應(yīng)該表示araDAB 缺失,但567實在不知道什么意思,是不是什么編號之類的? [ Last edited by 望月臺 on 2010-7-27 at 21:10 ] |

銅蟲 (小有名氣)

木蟲 (小有名氣)
木蟲 (正式寫手)
夢幻biobrick

銀蟲 (正式寫手)
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BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 (CGSC6159)[1], which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 [2], a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113. BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2]. According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter. References See Help:References for how to manage references in EcoliWiki. ↑ 1.0 1.1 1.2 1.3 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed EcoliWiki page ↑ 2.0 2.1 2.2 2.3 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page 詳細(xì)信息:http://ecoliwiki.net/colipedia/index.php/BW25113 PS:該菌株是日本慶應(yīng)義塾大學(xué)(Keio collection)常用于大腸桿菌基因敲除的野生型菌株(WT)。 |
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