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題目:Synthesis of γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications 摘要:Agriculture surplus were used as substrates to synthesize γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield and synthesis of GABA with respect to whey milk. Optimal conditions for synthesizing GABA in grape must were: initial pH 6.0, initial cell density of Log 7.0 cfu/mL, and addition of 18.4 mM L-glutamate. L. plantarum DSM19463 synthesized 4.83 mM of GABA during fermentation at 30°C for 72 h. The fermented grape must also contain various levels of niacin, free minerals, and polyphenols, and Log 10.0 cfu/g of viable cells of L. plantarum DSM19463. Freeze dried preparation of grape must was applied to the SkinEthic® Reconstructed Human Epidermis or multi-layer human skin model (FT-skin tissue). The effect on transcriptional regulation of human beta-defensin-2 (HBD-2), hyaluronan synthase (HAS1), filaggrin (FGR), and involucrin genes was assayed through RT-PCR. Compared to GABA used as pure chemical compound, the up-regulation HBD-2 was similar while the effect on the expression of HAS1 and FGR genes was higher. |
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題目::DSM19463乳酸乳桿菌合成的γ-氨基丁酸可以生產(chǎn)功能性葡萄汁飲料,也可用于皮膚疾病的治療 摘要:農(nóng)業(yè)產(chǎn)品葡萄的剩余,可作為DSM19463乳酸乳桿菌合成γ-氨基丁酸的底物以生產(chǎn)一種功能飲料來使用,或者被新穎的用于治療皮膚科的疾病。該葡萄汁必須被稀釋到總的碳水化合物的含量 1 or 4%(w/v),γ-氨基丁酸關(guān)于乳清蛋白的合成。在葡萄汁里合成γ-氨基丁酸最優(yōu)的條件是:初始的ph值為6,細(xì)胞濃度為Log7.0 cfu/mL,再添加18.4 mM L-谷氨酸。DSM19463乳酸乳桿菌 30°C 條件下發(fā)酵72小時可合成4.83 mM γ-氨基丁酸,已經(jīng)發(fā)酵的葡萄汁也含有不同含量的煙酸、游離的礦物質(zhì)、多酚以及含量為Log 10.0 cfu/g 的DSM19463乳酸乳桿菌的活細(xì)胞。凍干的葡萄汁也應(yīng)用與人類再生上皮細(xì)胞以及人類的皮膚模型,人體防御素-2、透明質(zhì)酸、絲聚蛋白以及包蛋白基因?qū)D(zhuǎn)錄的調(diào)節(jié)已經(jīng)通過反轉(zhuǎn)錄酶-聚合鏈酶實驗得到驗證。它作為單純的化合物,在上調(diào)人體防御素-2的作用上,凍干的葡萄汁跟γ-氨基丁酸比是相似的,然而在 HAS1 and FGR基因的表達(dá)上的作用上它則顯得更強。 |
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