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caojun2318金蟲(chóng) (小有名氣)
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[交流]
【求助/交流】細(xì)胞熒光標(biāo)記問(wèn)題求助 已有2人參與
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| 我想對(duì)不同細(xì)胞跨膜途徑的蛋白（網(wǎng)格蛋白，陷穴蛋白）進(jìn)行熒光標(biāo)記，但是查了很久沒(méi)有查到有哪種特異的熒光標(biāo)記物能對(duì)這兩種蛋白進(jìn)行標(biāo)記的，有誰(shuí)做過(guò)，或者知道這方面的工作？選用哪些熒光探針能特異標(biāo)記這些蛋白？ |
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上海生工有進(jìn)口試劑盒供應(yīng)！ HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine) 蛋白羅丹明標(biāo)記試劑盒 Description: (5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format. Features: Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15～-20℃ 編號(hào) 包裝 價(jià)格 產(chǎn)地 786-058 5 Reactions 2,486 GBios HOOK™ Dye Labeling Kit (FITC) 蛋白FITC熒光素標(biāo)記試劑盒 Description: FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. Features: Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15～-20℃ 編號(hào) 包裝 價(jià)格 產(chǎn)地 786-059 5 Reactions 2,486 GBios |
2樓2010-12-05 15:23:46
三磷酸腺苷
鐵桿木蟲(chóng) (職業(yè)作家)
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3樓2010-12-05 19:01:57
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