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[交流]
【求助/交流】請教小麥HSP70的一段序列
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| 我做過HSP70蛋白的western表達,有趨勢。所以想繼續(xù)從RNA水平做RT試試。但是沒有找到小麥HSP70的序列,無法設計引物,有高人知道么,希望不吝賜教,感恩不盡。嘿嘿 |
» 搶金幣啦!回帖就可以得到:
+5/535
+2/104
+1/78
+1/64
+1/37
+1/35
+2/26
+1/9
+1/7
+1/6
+1/5
+1/5
+1/4
+1/2
+1/2
+1/2
+1/1
+1/1
+1/1
+1/1
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非常感謝指點 我會好好看下這篇文章的 這個帖子發(fā)了很久了,不能加BB了,嘿嘿。 http://www.gaoyang168.com/bbs/viewthread.php?tid=2951781 這個我灌水的 可以追加 邀請你過來領紅包~~ 呼呼~~ ![]() ![]() |
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從NCBI上找就行,我?guī)湍憧戳艘幌率遣皇沁@個http://www.ncbi.nlm.nih.gov/gene/542817 http://www.ncbi.nlm.nih.gov/nuccore/6996622 |
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hsp70不是一個成員,應該有多個基因。你看看這兩個 AK332587.1| Triticum aestivum cDNA 145-2199 1 gaaggcctct cgccatttct cccttctctc aatcgcccaa aacccacgcg aatccaccag 61 ccccttcctt caacttcctg ccccttgtag ggtttcgccc cccaccaacc cctacccccc 121 gggttactgc tcgtgctctc agccatggcg accacgacct tccccacctc gacccccttc 181 ttcgtccatc acggcacccg gcgcccctcc gtcaacctcc gaacggccgc ggtggtgtac 241 ggccgtggtg ggcggcggtg gcggccactg cgcgtcgcct gcgagaaggt ggtggggatc 301 gacctcggga ccaccaactc agcggtcgcg gcgatggagg gcggcaagcc caccatcgtc 361 accaacgccg agggcgcgcg gaccacgccg tcggtcgtgg cgtacaccaa ggccggggac 421 cggctggtgg gacagatcgc caagaggcag gcggtggtca acccggagaa caccttcttc 481 tcagtcaaga ggttcatcgg ccgaaagatg aacgaggtcg ccgaagagtc caagcaggtt 541 tcctaccgcc ttgtccggga cgataacggc aacgtcaagc tcgattgccc agcaattggc 601 aagcagttcg ccgcggagga gatctctgca caggtcctga gaaagctggt tgatgatgca 661 tcaaagtttt taaatgacaa agtcaccaag gcagtgatca cagttcctgc ttatttcaat 721 gactcccaga ggacggctac aaaagatgcc ggccgcattg caggcctgga tgttctccgt 781 atcataaacg agcctaccgc ggcatcgttg gcatatggtt ttgaaaaaaa gaacaatgaa 841 acaattctgg tttttgacct gggaggaggc acctttgatg tttcagttct cgaagttggt 901 gatggtgttt ttgaggtgct gtctacatct ggtgatactc accttggcgg tgatgacttt 961 gacaagagaa ttgttgattg gctggctggg agcttcaaga atgatgaggg tattgacctg 1021 ctaaaagaca agcaagctct ccagcgtctt acagaagcag ctgagaaggc caagatggaa 1081 ttgtcatcgt tgacccaaac aaatatcagt ttacctttca ttacagctac tgctgatggg 1141 cccaagcata ttgagacaac acttaccagg ggtaaatttg aggagctatg ctcagatctt 1201 cttgacaggc tgaggacacc tgttgacaat tctcttagag atgcgaagtt gtcacttaaa 1261 gaaatagatg aggtgattct tgtgggtggt tccacaagaa ttccagctgt tcaggatctt 1321 gtgaagaaaa tgacgggaaa ggatcccaat gtgacagtca atcccgatga ggttgttgct 1381 cttggagcag cggtgcaggc aggagtattg tcgggcgatg tgagcgatat tgtgcttctt 1441 gatgttacgc cactgtctct tggtttggag acactgggtg ggatgatgac caagattatc 1501 ccaaggaaca caaccctccc tacctccaag tcagaggtct tttcaacggc tgctgatgga 1561 cagactagtg tggagattaa tgttctccaa ggagaaagag agtttgttag ggacaataaa 1621 tcccttggta gcttcaggct tgatggaatt cctcctgctc cgcgtggtgt tccccaaatt 1681 gaagtcaagt ttgatattga tgccaacggc attctgtctg ttgctgcggt ggataagggc 1741 actgggaaga agcaggacat tacgatcact ggagccagca cactgccaaa ggatgaggta 1801 gagaagatgg tggaagaagc tgaaaaattt gcggcggagg acaaggagaa gagggacgca 1861 attgacacca agaaccaggc agagtctgtg atgtaccaga ctgagaaaca actgaaggag 1921 ctcggagaca aggtcccagg cgatgtcaag gagaaggtgg aggggaagct caaggaactc 1981 caggatgccg ttgctggtgg atcgacacag acgataaagg atgcactagc tgcgttgaac 2041 caagaagtga tgcagcttgg tcagtccctc taccagcagc aggggtcggg ccctacccct 2101 ggagctgacg gcgctgctga ctctacaggc ccaggcccat ctgagaagac aggcgatggt 2161 ggtgacgtca tagacgctga ttttaccgac agcaattgaa tagaacagta ggttaggaca 2221 tgatgcaaca agtgagtatg aatttgttcc acaggattgc ctagcagtat acgggtgacc 2281 gttttctttg gattgagacc ttgtttgctg tcagggtgtt ggccgagttt ttgatgttga 2341 ggtttggaga cttcagttat cagtttggat gggatagatt ggcttttgct tgttgggaac 2401 ccgacccctt ggccttgtac ggttatgtat tggatttact aactctctag agaactgggt 2461 catttcctgt ttggagcatg tgccaaaaac tggattgtta ttttggggag cattctactc 2521 ccttcgttta taaatataaa gcccttttag agaaaaaaaa aaaaaaaaac ga Cds 1 ATGGCGACCA CGACCTTCCC CACCTCGACC CCCTTCTTCG TCCATCACGG CACCCGGCGC 61 CCCTCCGTCA ACCTCCGAAC GGCCGCGGTG GTGTACGGCC GTGGTGGGCG GCGGTGGCGG 121 CCACTGCGCG TCGCCTGCGA GAAGGTGGTG GGGATCGACC TCGGGACCAC CAACTCAGCG 181 GTCGCGGCGA TGGAGGGCGG CAAGCCCACC ATCGTCACCA ACGCCGAGGG CGCGCGGACC 241 ACGCCGTCGG TCGTGGCGTA CACCAAGGCC GGGGACCGGC TGGTGGGACA GATCGCCAAG 301 AGGCAGGCGG TGGTCAACCC GGAGAACACC TTCTTCTCAG TCAAGAGGTT CATCGGCCGA 361 AAGATGAACG AGGTCGCCGA AGAGTCCAAG CAGGTTTCCT ACCGCCTTGT CCGGGACGAT 421 AACGGCAACG TCAAGCTCGA TTGCCCAGCA ATTGGCAAGC AGTTCGCCGC GGAGGAGATC 481 TCTGCACAGG TCCTGAGAAA GCTGGTTGAT GATGCATCAA AGTTTTTAAA TGACAAAGTC 541 ACCAAGGCAG TGATCACAGT TCCTGCTTAT TTCAATGACT CCCAGAGGAC GGCTACAAAA 601 GATGCCGGCC GCATTGCAGG CCTGGATGTT CTCCGTATCA TAAACGAGCC TACCGCGGCA 661 TCGTTGGCAT ATGGTTTTGA AAAAAAGAAC AATGAAACAA TTCTGGTTTT TGACCTGGGA 721 GGAGGCACCT TTGATGTTTC AGTTCTCGAA GTTGGTGATG GTGTTTTTGA GGTGCTGTCT 781 ACATCTGGTG ATACTCACCT TGGCGGTGAT GACTTTGACA AGAGAATTGT TGATTGGCTG 841 GCTGGGAGCT TCAAGAATGA TGAGGGTATT GACCTGCTAA AAGACAAGCA AGCTCTCCAG 901 CGTCTTACAG AAGCAGCTGA GAAGGCCAAG ATGGAATTGT CATCGTTGAC CCAAACAAAT 961 ATCAGTTTAC CTTTCATTAC AGCTACTGCT GATGGGCCCA AGCATATTGA GACAACACTT 1021 ACCAGGGGTA AATTTGAGGA GCTATGCTCA GATCTTCTTG ACAGGCTGAG GACACCTGTT 1081 GACAATTCTC TTAGAGATGC GAAGTTGTCA CTTAAAGAAA TAGATGAGGT GATTCTTGTG 1141 GGTGGTTCCA CAAGAATTCC AGCTGTTCAG GATCTTGTGA AGAAAATGAC GGGAAAGGAT 1201 CCCAATGTGA CAGTCAATCC CGATGAGGTT GTTGCTCTTG GAGCAGCGGT GCAGGCAGGA 1261 GTATTGTCGG GCGATGTGAG CGATATTGTG CTTCTTGATG TTACGCCACT GTCTCTTGGT 1321 TTGGAGACAC TGGGTGGGAT GATGACCAAG ATTATCCCAA GGAACACAAC CCTCCCTACC 1381 TCCAAGTCAG AGGTCTTTTC AACGGCTGCT GATGGACAGA CTAGTGTGGA GATTAATGTT 1441 CTCCAAGGAG AAAGAGAGTT TGTTAGGGAC AATAAATCCC TTGGTAGCTT CAGGCTTGAT 1501 GGAATTCCTC CTGCTCCGCG TGGTGTTCCC CAAATTGAAG TCAAGTTTGA TATTGATGCC 1561 AACGGCATTC TGTCTGTTGC TGCGGTGGAT AAGGGCACTG GGAAGAAGCA GGACATTACG 1621 ATCACTGGAG CCAGCACACT GCCAAAGGAT GAGGTAGAGA AGATGGTGGA AGAAGCTGAA 1681 AAATTTGCGG CGGAGGACAA GGAGAAGAGG GACGCAATTG ACACCAAGAA CCAGGCAGAG 1741 TCTGTGATGT ACCAGACTGA GAAACAACTG AAGGAGCTCG GAGACAAGGT CCCAGGCGAT 1801 GTCAAGGAGA AGGTGGAGGG GAAGCTCAAG GAACTCCAGG ATGCCGTTGC TGGTGGATCG 1861 ACACAGACGA TAAAGGATGC ACTAGCTGCG TTGAACCAAG AAGTGATGCA GCTTGGTCAG 1921 TCCCTCTACC AGCAGCAGGG GTCGGGCCCT ACCCCTGGAG CTGACGGCGC TGCTGACTCT 1981 ACAGGCCCAG GCCCATCTGA GAAGACAGGC GATGGTGGTG ACGTCATAGA CGCTGATTTT 2041 ACCGACAGCA ATTGA TA GU452716 CDS 38..2110 /codon_start=1 /product="70 kDa heat shock protein" /protein_id="ADD23344.1" /db_xref="GI:290131414" /translation="MATFTSQVSAMAGASPSCSLFVSRRRPAVMPLQMRVARGGRPRG LAMRVTCEKVVGIDLGTTNSAVAAMEGGKPTVITNAEGQRTTPSVVAYTKGGERLVGQ IAKRQAVVNPVNTFFSVKRFIGRKMAEVDDEAKQVSYNVVRDENGNVKLDCPAIGKQF AAEEISAQVLRKLVDDASKFLNDKITKAVVTVPAYFNDSQRTATKDAGRIAGLEVLRI INEPTAASLAYGFEKKNNETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDD FDKKIVDWLASTFKNDEGIDLLKDKQALQRLTEAAEKAKMELSTLTQANISLPFITAT ADGPKHIEATLSRAKFEELCSDLIDRLKTPVNNALKDAKLSVSNLDEVILVGGSTRIP AVQETVRKITGKDPNVTVNPDEVVSLGAAVQGGVLAGDVKDVVLLDVTPLSIGLETLG GVMTKIIPRNTTLPTSKSEVFSTAADGQTSVEINVLQGEREFVRDNKSLGSFRLDGIP PAPRGVPQIEVKFDIDANGILSVAAVDKGTGKKQDITITGASTLPKDEVERMVEEADK FAQEDKEKRDAIDTKNQADSVVYQTEKQLKELGDKVPAPVKEKVDVKLQELKDAIAGG STQSMKTAMEALNQEVMQIGQAMYNQTSAGDAGSTDAETEPGAGSTSSGKGPNDGDVI DADFTDSN" ORIGIN 1 ttcactaggg ttagggttta cgtaacgaac ccgcgccatg gctaccttca cttcccaggt 61 cagcgcgatg gctggcgcct ccccgtcgtg ctctctcttc gtgagccggc ggcggccggc 121 ggtcatgccc ctgcagatgc gggttgctcg tggcgggagg ccgcgcgggt tggcgatgcg 181 ggtgacgtgc gagaaggtgg tgggaatcga cctgggcacc accaactccg ccgtcgcggc 241 gatggagggc ggtaagccga cggtgatcac caacgccgag ggccagcgga cgacgccgtc 301 ggtggtggcg tacaccaagg gaggagaacg gctagtgggc cagattgcca agaggcaggc 361 cgtcgtcaac ccggtgaaca ccttcttctc cgtcaagcgc ttcattggac gcaagatggc 421 tgaggtagat gacgaggcca agcaggtctc gtataacgtt gtgcgcgatg agaacggaaa 481 cgtcaagctc gactgccctg ctatcggcaa gcagttcgca gcggaagaga tatccgcgca 541 ggtattgagg aaattggtgg atgatgcatc taagtttctg aatgacaaaa ttacaaaagc 601 ggtggttacc gttccagcgt acttcaatga ctcgcagaga acagcaacaa aggatgctgg 661 ccgtattgca gggctggaag ttctgcgcat tataaatgag ccaactgctg catctcttgc 721 ttatggtttt gagaagaaaa acaatgaaac gattctagtg tttgacttgg gtggcggtac 781 ttttgatgtc tctgtattgg aggttggaga tggtgtattt gaggtgcttt ccacatctgg 841 ggacacacac ttaggtggag atgactttga taagaaaatt gtagattggc ttgctagcac 901 cttcaagaat gatgaaggca tcgatcttct taaggataaa caagcccttc agcgtcttac 961 tgaggcagca gaaaaagcta aaatggaatt gtcaacgctc acacaggcga acattagctt 1021 gccattcata actgctactg ctgatgggcc caaacacatt gaggcaaccc tctctagagc 1081 taaattcgag gaactgtgtt cagatctgat tgataggctt aaaactcctg ttaataacgc 1141 cttgaaagat gccaagctat ctgttagtaa tctagatgaa gtgattcttg tgggtggatc 1201 cacccgaatc cctgcagtgc aagaaactgt taggaagatt acaggcaagg atcccaatgt 1261 tacagtcaac cctgatgagg ttgtttctct tggggcggcc gtacagggtg gagttttggc 1321 tggagatgtg aaggatgttg ttcttcttga tgttactcca ttgtcaattg gtttggagac 1381 gttgggtgga gtgatgacga agattatccc caggaataca acactgccca cctccaaatc 1441 agaggtattc tcaacggctg ctgatggaca gacaagtgtt gagattaatg tgctccaggg 1501 agagagagag tttgtcaggg acaacaagtc tcttggaagc ttccgactgg atgggatccc 1561 tcctgcacca cgtggtgtac cacaaattga agtgaagttt gacattgatg ccaatggcat 1621 tctgtcagta gcagctgttg ataagggtac tggaaagaag caggatatca ccatcactgg 1681 tgctagtact ctaccaaagg atgaggtcga gagaatggta gaagaagctg acaagtttgc 1741 ccaggaggac aaagagaaaa gagatgctat tgacaccaag aaccaagcag actctgtggt 1801 gtaccagacg gagaagcaac tgaaggagct tggggacaag gtccctgctc ctgtgaaaga 1861 gaaggttgat gtaaagctcc aggaactgaa agatgccatt gctggtggat cgacacagag 1921 catgaaaact gccatggagg ctttgaacca ggaggtaatg cagatcggac aggctatgta 1981 caaccaaact agtgctggtg atgctggctc tactgatgct gaaactgagc ctggtgctgg 2041 atcaacaagc tcaggaaagg gaccaaatga tggagatgtt atcgatgcgg acttcacaga 2101 tagcaattaa aggatggtca atggatcgtg gtgagtgata catagaccct gagttatatt 2161 ctgagtggca ttctagggtt gaggatgtta tttttgtatc tcggctccat gtatctacaa 2221 gctgtttatt cagtatatga aatttggtag cggtaggact caacaacgtt tgctagagtg 2281 atcttgaatc aagtataccc ctctggtaat gcaaagatgc aaaaggattt tggtcgaaac 2341 agtagcatat tccctaaaaa gtaaactagc catgtttagg ttagcaataa acgaagtttc 2401 atggtgtaaa |
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