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北京石油化工學(xué)院2026年研究生招生接收調(diào)劑公告

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biomill8358

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[交流] 【分享】由MII卵母細(xì)胞高效誘導(dǎo)體細(xì)胞重編程

High-efficiency somatic reprogramming induced by intactMII oocytes
由MII卵母細(xì)胞高效誘導(dǎo)體細(xì)胞重編程
HuiYang1, LinyuShi1, ShenghuaZhang1, JiangweiLin1, Jing Jiang1, JinsongLi1
1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, 320 YueyangRoad, Shanghai 200031, China
Cell Research (2010) 20:1034-1042
摘要：Somatic nuclei can be reprogrammed into a pluripotent state by nuclear transfer, cell fusion and expression of transcription factors. However, these reprogramming processes are very inefficient, which has greatly hindered efforts to elucidate the underlying molecular mechanisms. Here, we report a new reprogramming strategy that combines the advantages of all three reprogramming methodologies into one process. We injected nuclei from cumulus cells into intact MII oocytes. Following activation, 80% of the reconstructed embryos developed to the blastocyst stage, and tetraploid (4N) embryonic stem (ES) cell lines were generated at a rate of 30% per reconstructed oocyte. We also generated triploid (3N) ES cells after injection of somatic nuclei into activated oocytes. 4N and 3N ES cells expressed pluripotent markers and differentiated into cell types of three embryonic germ layers in vivo. Moreover,all ES cells generated histocompatible, differentiated cells after being engrafted in immunocompetent B6D2F1 mice,showing that ES cells derived from this reprogramming strategy might serve as a source of genetically tailored tissues for transplantation. Thus, we have established a simple and highly efficient reprogramming procedure that provides a system for investigating the molecular mechanisms involved in somatic reprogramming.
1.這篇文章中，作者報道了集三種重編程方法的優(yōu)點于一體的新的重編程策略。作者將卵丘細(xì)胞的細(xì)胞核注射到完整的MII卵母細(xì)胞中。激活之后，有80%的重組的胚胎發(fā)育到了囊胚階段，并且每一個重組的卵母細(xì)胞形成4倍體的ES細(xì)胞的幾率是30%。
2.作者將體細(xì)胞核注射到激活的卵母細(xì)胞中后也獲得了3倍體的ES細(xì)胞。4N和3N的ES細(xì)胞都表達多功能標(biāo)記并且在體外能分化到三層胚胎。
3.所有的ES細(xì)胞在移入具有免疫活性的B6D2F1老鼠體內(nèi)都能產(chǎn)生組織相容性，分化的細(xì)胞。這表明用這種方法獲得的ES細(xì)胞可以用作組織移植的來源。作者為研究體細(xì)胞重編程的分子機制提供了一個簡單高校的系統(tǒng)。
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