 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 5 | 1/1 | 返回列表 |
| 查看: 1650 | 回復(fù): 13 | |||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | |||
| 當(dāng)前只顯示滿足指定條件的回帖，點(diǎn)擊這里查看本話題的所有回帖 | |||
[交流]
翻譯通順點(diǎn)
|
|||
| Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer. After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. |
回復(fù)此樓» 猜你喜歡
347求調(diào)劑
已經(jīng)有4人回復(fù)
-
一志愿西交288化工專碩求調(diào)劑
已經(jīng)有3人回復(fù)
-
一志愿中國科學(xué)院大學(xué)265求調(diào)劑
已經(jīng)有4人回復(fù)
-
291求調(diào)劑
已經(jīng)有13人回復(fù)
-
070300化學(xué)求調(diào)劑
已經(jīng)有3人回復(fù)
-
322求調(diào)劑
已經(jīng)有7人回復(fù)
-
0703化學(xué)
已經(jīng)有15人回復(fù)
-
材料學(xué)碩333求調(diào)劑
已經(jīng)有15人回復(fù)
-
313求調(diào)劑
已經(jīng)有3人回復(fù)
-
083000學(xué)碩274求調(diào)劑
已經(jīng)有11人回復(fù)
» 搶金幣啦！回帖就可以得到:
-
齊齊哈爾大學(xué)材料科學(xué)與工程學(xué)院 誠招調(diào)劑生
+1/986
-
2026級藥學(xué)學(xué)碩調(diào)劑招生
+1/292
-
【招聘】溫州醫(yī)科大學(xué)/附屬醫(yī)院/甌江實(shí)驗(yàn)室李校堃院士團(tuán)隊(duì)誠聘博士后和科研人員
+1/185
-
山東建筑大學(xué) （市政與環(huán)境工程學(xué)院）碩士研究生招生啦 靜電紡絲纖維與鹽水分離應(yīng)用
+1/85
-
福建師范大學(xué)招收2026年化學(xué)、材料碩士3-4名
+1/84
-
南方科技大學(xué)王鵬團(tuán)隊(duì)誠聘藥物化學(xué)、化學(xué)探針、藥物遞送博士后
+1/79
-
武漢紡織大學(xué)全重實(shí)驗(yàn)室國家級人才團(tuán)隊(duì)先進(jìn)纖維與功能器件課題組 接收調(diào)劑研究生
+1/49
-
2026延安大學(xué)設(shè)施蔬菜環(huán)境調(diào)控與分子機(jī)制研究團(tuán)隊(duì)團(tuán)隊(duì)招收相關(guān)專業(yè)調(diào)劑研究生
+1/38
-
武漢工程大學(xué)材料學(xué)院課題組碩士調(diào)劑招生
+1/38
-
華南理工大學(xué)周奕彤老師課題組招收2026級博士生-外骨骼機(jī)器人
+1/35
-
長安大學(xué)智能爆破課題組招收2026級博士和碩士研究生
+1/20
-
上海中醫(yī)藥大學(xué)創(chuàng)新中藥研究院 招收審核制博士生一名
+1/16
-
福建農(nóng)林大學(xué)生物質(zhì)能源與材料研究中心調(diào)劑（08工科，考數(shù)學(xué)的來）
+1/10
-
博士招生 | 青島科技大學(xué)（高分子、化學(xué)、材料、力學(xué)、機(jī)電工程、計(jì)算機(jī)仿真）
+1/6
-
北京石油化工學(xué)院VOCs凈化技術(shù)課題組 招收2026級碩士調(diào)劑
+1/4
-
2026年上海工程技術(shù)大學(xué)材料學(xué)院智能材料與器件課題組招收碩士調(diào)劑生。
+1/3
-
2026年江西科技師范大學(xué)Tide課題組招收化學(xué)、生物、藥學(xué)、材料相關(guān)學(xué)碩或?qū)４T調(diào)劑生
+1/3
-
2026年北京石油化工學(xué)院環(huán)境學(xué)科-水污染控制工程方向課題組歡迎您
+1/2
-
武漢紡織大學(xué)沈愛國教授團(tuán)隊(duì)招收化學(xué)、生物、材料專業(yè)研究生
+1/1
-
廣東唯一石化院校——資源與環(huán)境專碩招生
+1/1
沒辦法啊，老師要讓翻譯，自己不會(huì)，只能求助別人了，唉，太難了，
7樓2011-03-20 22:30:06
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號: 1150928
昔年殘夢(金幣+10, 翻譯EPI+1): 2011-03-20 22:44:44
|
Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer 根據(jù)1979年Boller 等進(jìn)行的ACC合成酶的提取物（ACS）的活性測定并經(jīng)Lizada和楊（1979）修改過后的準(zhǔn)備方法如下：將1g番茄果皮組織通過一個(gè)冷的杵和研缽進(jìn)行研磨，直到將其研磨成勻漿。在研磨過程中加入5毫升的提取緩沖液，其中包含有4毫摩二硫蘇糖醇，10毫摩磷酸吡哆醛，加有1毫摩EDTA的100毫摩、PH為8的HEPES緩沖液。 |
2樓2011-03-20 22:20:14
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號: 1150928
3樓2011-03-20 22:20:33
Mally89
鐵桿木蟲 (職業(yè)作家)
- 翻譯EPI: 79
- 應(yīng)助: 7 (幼兒園)
- 貴賓: 1.257
- 金幣: 5347.3
- 帖子: 3712
- 在線: 1225.5小時(shí)
- 蟲號: 1150928
|
After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. 將上述勻漿在10000轉(zhuǎn)20分鐘離心后，取上清液用于酶檢測。整個(gè)酶提取過程都應(yīng)該在4度環(huán)境下進(jìn)行。 ACC合成酶的活性需要放入裝有0.8mL的反應(yīng)復(fù)合物的試管中進(jìn)行測定，此復(fù)合物含0.25毫摩S -腺苷甲硫氨酸，50毫摩HEPES緩沖液和10毫摩帶有0.2ml酶提取物的磷酸吡哆醛。 |
5樓2011-03-20 22:27:26
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 食品工程專碩一志愿中海洋309求調(diào)劑 +5 | 小張zxy張 2026-03-26 | 10/500 |
|
|---|---|---|---|---|
|
|
[考研] 266分，求材料相關(guān)專業(yè)調(diào)劑 +6 | 哇呼哼呼哼 2026-03-30 | 8/400 |
|
|
[考研] 288資源與環(huán)境專碩求調(diào)劑，不限專業(yè)，有學(xué)上就行 +11 | lllllos 2026-03-30 | 11/550 |
|
|
|
[考研] 286分調(diào)劑 +10 | Faune 2026-03-30 | 11/550 |
|
|
|
[考研] 311求調(diào)劑 +7 | 藍(lán)月亮亮 2026-03-30 | 7/350 |
|
|
|
[考研] 297求調(diào)劑 +17 | 田洪有 2026-03-26 | 18/900 |
|
|
|
[考研] 本科211生物醫(yī)學(xué)工程085409求調(diào)劑339分 +3 | 里子木yy 2026-03-29 | 3/150 |
|
|
|
[考研] 環(huán)境科學(xué)與工程334分求調(diào)劑 +6 | 王一一依依 2026-03-30 | 8/400 |
|
|
|
[考研] 南京大學(xué)化學(xué)調(diào)劑 +10 | 景隨風(fēng) 2026-03-29 | 15/750 |
|
|
|
[考研] 275求調(diào)劑 +15 | Micky11223 2026-03-25 | 20/1000 |
|
|
|
[考研] 【求調(diào)劑】085601材料工程專碩 | 總分272 | +7 | 腳滑的守法公民 2026-03-27 | 7/350 |
|
|
|
[考研] 291求調(diào)劑 +5 | Y-cap 2026-03-29 | 6/300 |
|
|
|
[考研] 332求92調(diào)劑 +8 | 蕉蕉123 2026-03-28 | 8/400 |
|
|
|
[考研] 調(diào)劑求院校招收 +6 | 鶴鯨鴿 2026-03-28 | 6/300 |
|
|
|
[考研] 311（085601）求調(diào)劑 +4 | liziyeyeye 2026-03-28 | 4/200 |
|
|
|
[考研] 283求調(diào)劑 +3 | A child 2026-03-28 | 3/150 |
|
|
|
[考研] 266求調(diào)劑 +11 | 陽陽哇塞 2026-03-27 | 12/600 |
|
|
|
[考研] 348求調(diào)劑 +4 | 小懶蟲不懶了 2026-03-27 | 5/250 |
|
|
|
[考研] 考研調(diào)劑 +10 | 呼呼？~+123456 2026-03-24 | 10/500 |
|
|
|
[考研] 286求調(diào)劑 +4 | lim0922 2026-03-26 | 4/200 |
|