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liying841216金蟲 (初入文壇)
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[求助]
300金幣求助翻譯小碩論文摘要
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| 背景與目的:實(shí)驗(yàn)通過建立模擬人皮膚取皮區(qū)創(chuàng)面來檢測(cè)生長(zhǎng)因子TGF-β1和VEGF在創(chuàng)面愈合以及瘢痕增生方面的作用,為臨床應(yīng)用新型藻酸鹽敷料治療取皮區(qū)創(chuàng)面的應(yīng)用提供數(shù)據(jù)依據(jù),方法:主要將藻酸鹽敷料應(yīng)用于版納微型豬近交系中厚皮取皮區(qū)創(chuàng)面,通過檢測(cè)不同時(shí)間點(diǎn)(取皮后0、3、7、14、28天)創(chuàng)面愈合過程中及創(chuàng)面愈合后的生長(zhǎng)因子TGF-β1、VEGF的mRNA表達(dá)情況,探討新型藻酸鹽敷料對(duì)創(chuàng)面愈合的生物學(xué)機(jī)制及減輕瘢痕增生的具體作用機(jī)制。結(jié)果:構(gòu)建的取皮區(qū)創(chuàng)面動(dòng)物模型,從大體外觀上觀測(cè),其藻酸鹽治療組與凡士林對(duì)照組存在明顯差異,治療組創(chuàng)面愈合時(shí)間少于對(duì)照組(P<0.05),具有統(tǒng)計(jì)學(xué)差異。取皮區(qū)創(chuàng)面愈合過程中不同時(shí)間點(diǎn)的治療組與對(duì)照組的基因表達(dá)情況表明,不同的時(shí)間點(diǎn)之間有顯著差異(P<0.01),治療與否有顯著性差異(P=0.025),不同豬之間無統(tǒng)計(jì)學(xué)差異。TGF-β1在0、3、7、14、28天的表達(dá)量為:治療組-5.068±0.286,-4.131±0.396,-3.294±0.516,-3.514±0.374,-4.695±0.809;對(duì)照組-5.068±0.286,-4,457±0.450,-4.262±0.512,-4.025±0.365,-4.485±0.233。TGF-β1表現(xiàn)為隨著創(chuàng)面的愈合其表達(dá)上升,至中期表達(dá)升至最高,之后表達(dá)下降,在創(chuàng)面完全愈合后其表達(dá)量趨于正常水平。其中在14天藻酸鹽治療組與凡士林對(duì)照組之間TGF-β1表達(dá)有顯著差異。VEGF的mRNA表達(dá)量的統(tǒng)計(jì)結(jié)果與TGF-β1的結(jié)果相似,其中7天藻酸鹽治療組與凡士林對(duì)照組之間VEGF的mRNA的表達(dá)有顯著差異。VEGF在0、3、7、14、28天的表達(dá)量為:治療組-3.461±0.279,-2.726±0.425,-1.722±0.097,-2.401±0.341,-2.902±0.437,對(duì)照組為-2.928±0.385,-2.522±0.319,-2.877±0.517,-3.230±0.451。結(jié)論:通過構(gòu)建取皮后中厚皮創(chuàng)面動(dòng)物模型,成功構(gòu)建了檢測(cè)取皮區(qū)創(chuàng)面組織中VEGF、TGF-β1的mRNA的SYBR GreenⅠ實(shí)時(shí)熒光定量檢測(cè)方法,揭示藻酸鹽敷料對(duì)促進(jìn)創(chuàng)面愈合有重要作用,且主要與調(diào)控細(xì)胞因子的表達(dá)相關(guān),為解決創(chuàng)面愈合減少瘢痕增生等臨床棘手問題提供了可靠的理論依據(jù)。 |
銅蟲 (初入文壇)
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銅蟲 (初入文壇)
| Background and purpose: experiments to simulate the skin by establishing take skin area to detect growth factor wound TGF - beta 1 and VEGF in wound healing and the role of scar tissue hyperplasia for clinical application, new alginate dressings treatment take skin area wound applied to provide data basis, methods: main will alginate dressing applied in miniature pig with coefficient of thick skin take skin area through testing the tissue, at different time points (0, 3 after take skin, 7, 14 and 28 days) coagulant process and the growth factor coagulant TGF - beta 1, VEGF, discusses the mRNA expression of new alginate dressing coagulant biological mechanism and reduce the specific mechanism scar hyperplasia. Results: the construction of animal model take skin area wound from the gross appearance on alginate observation, the treatment group and control group vaseline, there exists significant difference in treatment group wound healing time less than in control group (P < 0.05), has a statistically significant. Take skin area coagulant at different time points in the process of the treatment group and control group, gene expression in different time points that there was a significant difference between (P < 0.01), treatment or not have significant difference (P = 0.025), between different pig was not statistically significant. TGF - beta 1 at 0, 3, 7, 14 and 28 days of express quantity is: treatment group - 5.068 + 4.131 + 0.396 0.286, - 0.516, 3.294 +, - 0.374 3.514 +, - - 4.695 + 0.809; 5.068 + 0.286 group - 0.450 4,457 +, - 0.512, 4.262 +, - 0.365 4.025 +, - - 4.485 + 0.233. TGF - beta 1 performance as the healing of the wound up its expression, to medium express to its hi |
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