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請高人幫忙修改一下語法錯誤，因為是一段Abstract，所以還是比較重視。 多謝了。bb可以增加的。 A polysaccharide was isolated from herb-A using ultrasonic-assisted extraction, and then further purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to afford P-1 and P-2. Structural characterizations of P-1 and P-2 (including molecular weight, monosaccharides composition, sulphate and uronic acid contents, conformational structure, ultraviolet spectrum, and infrared spectrum) were investigated, and their anti-proliferation activities against human gastric cancer BGC-823 cells and lung cancer A549 cells were researched in vitro. Results suggested that both P-1 and P-2 could significantly suppress the proliferation of BGC-823 cells in a concentration-dependent manner. The highest inhibitory rates were 78% for P-1 and 95% for P-2 at the concentration of 200 μg/ml, respectively. This high anticancer activity was inferred to be owing to the structural characterizations of polysaccharide, as well as the recognition of biological system for the conformational structure of polysaccharides. |
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zuodg
鐵桿木蟲 (正式寫手)
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2樓2011-06-22 00:30:48
zuodg
鐵桿木蟲 (正式寫手)
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【答案】應(yīng)助回帖
★
sltmac(金幣+1): 3Q 2011-06-22 22:06:57
sltmac: 人家讓改一下語法錯誤。。。。。。。。。。。 2011-06-27 10:25:50
伊人笑(金幣+1): 錯了大哥 2011-06-27 21:02:32
sltmac(金幣+1): 3Q 2011-06-22 22:06:57
sltmac: 人家讓改一下語法錯誤。。。。。。。。。。。 2011-06-27 10:25:50
伊人笑(金幣+1): 錯了大哥 2011-06-27 21:02:32
| 超聲輔助萃取法從草藥A中分離多糖。進一步用DEAE纖維素-52色譜柱與Sephadex G-100分子排阻色譜柱純化獲得P-1與P-2。對P-1與P-2進行了分子鑒定，包括分子量，單糖組成，硫酸與糖醛酸含量，構(gòu)象，紫外譜以及紅外譜。進行了體外抗增生活性實驗，細(xì)胞株是人腸道癌BGC-823細(xì)胞與肺癌A549細(xì)胞。結(jié)果顯示，依據(jù)濃度高低，P-1，P-2能夠顯著抑制BGC-823細(xì)胞的增生。P-1，P-2濃度分別達(dá)到200 μg/ml時，抑制效率達(dá)到最大，78%。如此之高的抗癌活性可能與多糖化學(xué)結(jié)構(gòu)以及生物系統(tǒng)對多糖構(gòu)象的識別有關(guān)。 |
3樓2011-06-22 00:38:39
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至尊木蟲 (職業(yè)作家)
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★
sltmac(金幣+1): 3Q 2011-06-22 22:07:04
伊人笑(金幣+5): 多謝。。。。。。 2011-06-27 21:02:10
sltmac(金幣+6): 謝謝交流~ 2011-06-30 08:41:17
sltmac(金幣+1): 3Q 2011-06-22 22:07:04
伊人笑(金幣+5): 多謝。。。。。。 2011-06-27 21:02:10
sltmac(金幣+6): 謝謝交流~ 2011-06-30 08:41:17
| Polysaccharides were isolated from herb-A with ultrasonic-assisted extraction, and then purified through DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to obtain P-1 and P-2. Structural characteristics of P-1 and P-2 including molecular weight, monosaccharides composition, sulphate and uronic acid contents, conformational structure, ultraviolet spectrum and infrared spectrum and their anti-proliferation activities against human gastric cancer BGC-823 cells and lung cancer A549 cells in vitro were investigated. It is suggested that both P-1 and P-2 can significantly suppress the proliferation of BGC-823 cells in a concentration-dependent manner. The highest inhibitory rates were 78% for P-1 and 95% for P-2 at the concentration of 200 μg/ml, respectively. This high anticancer activity can be referred to the structural characteristics of polysaccharide and especially the recognition of biological system to the conformational structure of polysaccharides. |
4樓2011-06-22 09:00:03
5樓2011-06-23 22:27:13
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★
sltmac(金幣+1): 謝謝應(yīng)助~ 2011-06-27 10:26:58
伊人笑(金幣+2, 翻譯EPI+1): 多謝，。。。。。。 2011-06-27 21:01:48
sltmac(金幣+6): 感謝應(yīng)助~ 2011-06-30 08:41:29
sltmac(金幣+1): 謝謝應(yīng)助~ 2011-06-27 10:26:58
伊人笑(金幣+2, 翻譯EPI+1): 多謝，。。。。。。 2011-06-27 21:01:48
sltmac(金幣+6): 感謝應(yīng)助~ 2011-06-30 08:41:29
| A polysaccharide ,from which can sepratede P-1 and P-2 by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography, was extracted from herb-A using ultrasonic-assisted extraction. Then identify the Structural characterizations of P-1 and P-2 ,including molecular weight, monosaccharides composition, sulphate and uronic acid contents, conformational structure, ultraviolet spectrum, and infrared spectrum, and study their anti-proliferation activities against human gastric cancer BGC-823 cells and lung cancer A549 cells in vitro. It can be seen from the research that both P-1 and P-2 can significantly inhibit the proliferation of BGC-823 cells in a certain concentration. The highest inhibitory rates were 78% for P-1 and 95% for P-2 at the concentration of 200 μg/ml. This higher anticancer activity may be attribute to the structural characterization of polysaccharides, and the recognition of biological system for the conformational structure of polysaccharides as well . |
6樓2011-06-24 09:40:43
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