| 3 | 1/1 | 返回列表 |
| 查看: 491 | 回復: 2 | ||
| 本帖產生 1 個 翻譯EPI ,點擊這里進行查看 | ||
[求助]
3句話生物專業(yè)漢譯英先謝謝了
|
||
| 目前關于酶分離純化的報道幾乎都以柱分離為主;钚詶l帶顯色方法主要是通過長波紫外線下檢測酮或者通過作用苷,再與與固藍B作用形成有色染料后檢測。還有其他染色方法如利用對酶特異性的單克隆抗體的免疫印跡法以及氧化氨乙基咔唑等。盡管上述方法能檢測酶,但是操作較為繁瑣,不易于分離純化研究,本研究選擇as做底物,通過形成的產物堿性條件下顯色原理,直接通過對照平板,從PAGE膠條中分離純化酶,達到省時省力,高效快速分離純化的目的。 |

鐵桿木蟲 (知名作家)

鐵桿木蟲 (知名作家)
| At present, nearly all reports on the purification of the enzyme are about the separation column. The main way of coloring the activity bands is either from detecting ketones through long-wave ultraviolet light or from detecting the colored dye formed by the interaction of the role of glycosides and fast blue B. There are also some other staining methods such as the western blot (WB) test of monoclonal antibody specific to the enzyme, the oxidation of aminoethyl carbazole and so on. Though the above methods can detect the enzyme, they are not good enough for the study of its purification due to the complicated operating procedures. Our study,however, chose as as the substrate and applied the theory of coloring the product under alkaline condition. We made the separation and purification of the enzyme from PAGE directly from the control panel. In this way, we achieved rapidly and efficiently the goal with less time and effort. |

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 318求調劑 +3 | plum李子 2026-03-23 | 3/150 |
|
|---|---|---|---|---|
|
[考研] 289材料與化工(085600)B區(qū)求調劑 +4 | 這么名字咋樣 2026-03-22 | 5/250 |
|
|
[考研] 調劑 +4 | 13853210211 2026-03-24 | 4/200 |
|
|
[材料工程] 一志愿C9材料與化工專業(yè)總分300求調劑 +4 | 曼111 2026-03-24 | 5/250 |
|
|
[考研] 284求調劑 +3 | yanzhixue111 2026-03-23 | 6/300 |
|
|
[考研] 333求調劑 +3 | ALULU4408 2026-03-23 | 3/150 |
|
|
[考研] 333求調劑 +6 | 87639 2026-03-21 | 10/500 |
|
|
[考研] 323求調劑 +6 | 洼小桶 2026-03-18 | 6/300 |
|
|
[考研] 一志愿西安交通大學材料工程專業(yè) 282分求調劑 +11 | 楓橋ZL 2026-03-18 | 13/650 |
|
|
[考研] 一志愿華中農業(yè)071010,總分320求調劑 +5 | 困困困困坤坤 2026-03-20 | 6/300 |
|
|
[考研] 306求調劑 +5 | 來好運來來來 2026-03-22 | 5/250 |
|
|
[考研] 一志愿中南化學(0703)總分337求調劑 +9 | niko- 2026-03-19 | 10/500 |
|
|
[考研] 324求調劑 +6 | lucky呀呀呀鴨 2026-03-20 | 6/300 |
|
|
[考研] 319求調劑 +4 | 小力氣珂珂 2026-03-20 | 4/200 |
|
|
[考研] 298求調劑一志愿211 +3 | 上岸6666@ 2026-03-20 | 3/150 |
|
|
[考研] 材料學碩301分求調劑 +7 | Liyouyumairs 2026-03-21 | 7/350 |
|
|
[考研] 0703化學297求調劑 +3 | Daisy☆ 2026-03-20 | 3/150 |
|
|
[考研] 266求調劑 +3 | 哇呼哼呼哼 2026-03-20 | 3/150 |
|
|
[考研] 求調劑 +3 | .m.. 2026-03-21 | 4/200 |
|
|
[考研] 308求調劑 +3 | 阿姐阿姐家啊 2026-03-18 | 3/150 |
|