 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 3 | 1/1 | 返回列表 |
| 查看: 493 | 回復(fù): 2 | ||
| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
[求助]
3句話生物專(zhuān)業(yè)漢譯英先謝謝了
|
||
| 目前關(guān)于酶分離純化的報(bào)道幾乎都以柱分離為主。活性條帶顯色方法主要是通過(guò)長(zhǎng)波紫外線下檢測(cè)酮或者通過(guò)作用苷，再與與固藍(lán)B作用形成有色染料后檢測(cè)。還有其他染色方法如利用對(duì)酶特異性的單克隆抗體的免疫印跡法以及氧化氨乙基咔唑等。盡管上述方法能檢測(cè)酶，但是操作較為繁瑣，不易于分離純化研究，本研究選擇as做底物，通過(guò)形成的產(chǎn)物堿性條件下顯色原理，直接通過(guò)對(duì)照平板，從PAGE膠條中分離純化酶，達(dá)到省時(shí)省力，高效快速分離純化的目的。 |
回復(fù)此樓» 猜你喜歡
295材料工程專(zhuān)碩求調(diào)劑
已經(jīng)有3人回復(fù)
-
085701環(huán)境工程求調(diào)劑
已經(jīng)有5人回復(fù)
-
083000學(xué)碩274求調(diào)劑
已經(jīng)有4人回復(fù)
-
275求調(diào)劑
已經(jīng)有8人回復(fù)
-
295求調(diào)劑
已經(jīng)有4人回復(fù)
-
一志愿211院校 344分 東北農(nóng)業(yè)大學(xué)生物學(xué)學(xué)碩，求調(diào)劑
已經(jīng)有8人回復(fù)
-
085602 307分 求調(diào)劑
已經(jīng)有5人回復(fù)
-
322求調(diào)劑
已經(jīng)有3人回復(fù)
-
高溫高壓反應(yīng)求助
已經(jīng)有3人回復(fù)
-
一志愿上海理工能源動(dòng)力（085800）310分求調(diào)劑
已經(jīng)有4人回復(fù)
Carena
鐵桿木蟲(chóng) (知名作家)
- 翻譯EPI: 94
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.542
- 金幣: 6371.2
- 散金: 8657
- 紅花: 55
- 沙發(fā): 23
- 帖子: 5968
- 在線: 357.5小時(shí)
- 蟲(chóng)號(hào): 1049282
- 注冊(cè): 2010-06-29
- 性別: MM
- 專(zhuān)業(yè): 外國(guó)語(yǔ)言
2樓2011-08-01 22:31:39
Carena
鐵桿木蟲(chóng) (知名作家)
- 翻譯EPI: 94
- 應(yīng)助: 5 (幼兒園)
- 貴賓: 0.542
- 金幣: 6371.2
- 散金: 8657
- 紅花: 55
- 沙發(fā): 23
- 帖子: 5968
- 在線: 357.5小時(shí)
- 蟲(chóng)號(hào): 1049282
- 注冊(cè): 2010-06-29
- 性別: MM
- 專(zhuān)業(yè): 外國(guó)語(yǔ)言
【答案】應(yīng)助回帖
★ ★
sltmac(金幣+2): 獎(jiǎng)勵(lì)~~ 2011-08-02 08:51:25
qwky2010(金幣+40, 翻譯EPI+1): 謝謝啊 2011-08-02 09:53:06
sltmac(金幣+2): 獎(jiǎng)勵(lì)~~ 2011-08-02 08:51:25
qwky2010(金幣+40, 翻譯EPI+1): 謝謝啊 2011-08-02 09:53:06
| At present, nearly all reports on the purification of the enzyme are about the separation column. The main way of coloring the activity bands is either from detecting ketones through long-wave ultraviolet light or from detecting the colored dye formed by the interaction of the role of glycosides and fast blue B. There are also some other staining methods such as the western blot (WB) test of monoclonal antibody specific to the enzyme, the oxidation of aminoethyl carbazole and so on. Though the above methods can detect the enzyme, they are not good enough for the study of its purification due to the complicated operating procedures. Our study,however, chose as as the substrate and applied the theory of coloring the product under alkaline condition. We made the separation and purification of the enzyme from PAGE directly from the control panel. In this way, we achieved rapidly and efficiently the goal with less time and effort. |
3樓2011-08-01 23:32:33
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 qwky2010 的主題更新
| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿南昌大學(xué)324求調(diào)劑 +3 | hanamiko 2026-03-27 | 3/150 |
|
|---|---|---|---|---|
|
|
[考研] 305求調(diào)劑 +5 | 哇盧卡庫(kù) 2026-03-26 | 5/250 |
|
|
|
[考研] 一志愿211，335分，0856，求調(diào)劑院校和導(dǎo)師 +4 | 傾____蕭 2026-03-27 | 5/250 |
|
|
[考研]
 材料與化工考研調(diào)劑
+16
|
孅華 2026-03-22 | 16/800 |
|
|
|
[考研] 324求調(diào)劑 +5 | hanamiko 2026-03-26 | 5/250 |
|
|
|
[考研] 304求調(diào)劑 +3 | 曼殊2266 2026-03-27 | 3/150 |
|
|
|
[考研] 324求調(diào)劑 +8 | hanamiko 2026-03-26 | 10/500 |
|
|
|
[考研] 286求調(diào)劑 +13 | Faune 2026-03-21 | 13/650 |
|
|
|
[考研] 材料考研求調(diào)劑 +3 | Dendel 2026-03-23 | 6/300 |
|
|
|
[考研] 303求調(diào)劑 +6 | 藍(lán)山月 2026-03-25 | 6/300 |
|
|
|
[考研] 296求調(diào)劑 +4 | 汪��？！ 2026-03-25 | 7/350 |
|
|
|
[考研] 086003食品工程求調(diào)劑 +6 | 淼淼111 2026-03-24 | 6/300 |
|
|
|
[考研] 上海電力大學(xué)材料防護(hù)與新材料重點(diǎn)實(shí)驗(yàn)室招收調(diào)劑研究生（材料、化學(xué)、電化學(xué)，環(huán)境） +4 | 我愛(ài)學(xué)電池 2026-03-23 | 4/200 |
|
|
|
[考研] 292求調(diào)劑 +4 | 鵝鵝鵝額額額額?/a> 2026-03-24 | 4/200 |
|
|
|
[考研] 333求調(diào)劑 +3 | ALULU4408 2026-03-23 | 3/150 |
|
|
|
[考研] 276求調(diào)劑 +3 | YNRYG 2026-03-21 | 4/200 |
|
|
|
[考研]
 一志愿東華大學(xué)化學(xué)070300，求調(diào)劑
+7
|
2117205181 2026-03-21 | 8/400 |
|
|
|
[考研] 求調(diào)劑院校信息 +6 | CX 330 2026-03-21 | 6/300 |
|
|
|
[考研] 考研調(diào)劑 +3 | 呼呼？~+123456 2026-03-21 | 3/150 |
|
|
|
[考研] 一志愿南大，0703化學(xué)，分?jǐn)?shù)336，求調(diào)劑 +3 | 收到VS 2026-03-21 | 3/150 |
|