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分子生物學(xué)-The PCR Revolution
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Contributors page vii Foreword by Russell Higuchi xi Preface xiii I BASIC TECHNOLOGIES 1 Real-time polymerase chain reaction 3 Mickey Williams 2 Thermostable enzymes used in polymerase chain reaction 12 Sudip K. Rakshit 3 Inventing molecular beacons 19 Fred Russell Kramer, Salvatore A. E. Marras, and Sanjay Tyagi 4 Rapid polymerase chain reaction and melting analysis 48 Carl T. Wittwer, Randy P. Rasmussen, and Kirk M. Ririe 5 Polymerase chain reaction and fluorescence chemistries: deoxyribonucleic acid incarnate 70 Ben Sowers 6 Analysis of microribonucleic acid expression by quantitative real-time polymerase chain reaction 80 Vladimir Benes, Jens Stolte, David Ibberson, Mirco Castoldi, and Martina Muckenthaler 7 Miniaturized polymerase chain reaction for quantitative clinical diagnostics 88 Melissa Mariani, Lin Chen, and Philip J. Day 8 The road from qualitative to quantitative assay: What is next? 110 Michael W. Pfaffl 9 Taking control of the polymerase chain reaction 129 Tania Nolan, Tanya Novak, and Jim Huggett II APPLICATIONS 10 Polymerase chain reaction–based methods for the detection of solid tumor cancer cells for clinical diagnostic and prognostic assays 155 Susan A. Burchill v 11 Polymerase chain reaction and infectious diseases 173 Jim Huggett 12 Polymerase chain reaction and respiratory viruses 189 Ian M. Mackay 13 Polymerase chain reaction and severe acute respiratory syndrome 212 Weijun Chen and Yang Huanming 14 The MMR vaccine, measles virus, and autism – A cautionary tale 229 Stephen A. Bustin 15 Noninvasive prenatal diagnosis using cell-free fetal nucleic acids in maternal plasma 243 Y. M. Dennis Lo 16 Polymerase chain reaction–based analyses of nucleic acids from archival material 254 Ulrich Lehmann 17 Microarrays and quantitative real-time reverse transcriptase–polymerase chain reaction 262 Elisa Wurmbach 18 Polymerase chain reaction in the detection of genetic variation 276 Pui-Yan Kwok 19 Polymerase chain reaction: A blessing and a curse for ancient deoxyribonucleic acid research 284 Michael Hofreiter and Holger Ro¨mpler Index 301 Color Plates follow page 214 |
PCR技術(shù)合輯 |
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