| 2 | 1/1 | 返回列表 |
| 查看: 440 | 回復(fù): 1 | |||
[交流]
求助,這個研究計(jì)劃應(yīng)該怎么寫?? 已有1人參與
|
|
求助各位大俠,老師讓寫個研究計(jì)劃,可是無處下手啊,之前不是學(xué)分子生物學(xué)的,請各位給一點(diǎn)建議,研究計(jì)劃的提綱如下: Regarding to your application, can you write a research proposal relevant to Wnt pathway? I would propose the following topic for your research proposal: Developing cell-based assay for Wnt signaling. The outline will be as followed: 1. Construct a vector encoding Frizzled receptor (a GPCR). Please include the detailed information of choosing the vetor, designing the primers for PCR and the whole cloning process, etc. 2. Construct a vector encoding report gene containing TCF-LEF responsive element (luciferase report gene or GFP gene) Please include the detailed information of choosing the vetor, designing the primers for PCR and the whole cloning process, etc. 3. Generate a cell line stably expressing the above two genes. Please include the process of stable cell line generation. 4. Expressing Wnt protein in bacteria expression system with affinity tag (His tag or GST tag) and purification of Wnt proteins. Please include the detailed information of choosing the vetor, designing the primers for PCR, the whole cloning process and protein purification, etc. 5. Verify the cell-based assay and carry out the protein activation assay During the writing process, you could refer to the webpage http://www.stanford.edu/group/nusselab/cgi-bin/wnt/ and check out the relevant literature. 萬分感激! |
木蟲 (職業(yè)作家)
哲學(xué)@草根
|
怎么會無處下手? 以第一個為例 事情:建載體 目的基因:Frizzled receptor (a GPCR) 什么物種? 人物:你自己,可問師兄師姐 過程:什么叫Frizzled receptor (a GPCR), 得查文獻(xiàn),用www.google.com查,或者學(xué)校數(shù)據(jù)庫,實(shí)在不懂的話,就先看中文的理解,后再看英文的。 研究計(jì)劃撰寫:1 為什么要建該載體,意義何在 2 關(guān)于連在載體上該基因的相關(guān)研究進(jìn)展 3 如何建, 別人問獻(xiàn)可以參考。 要記。喝绻裁炊伎梢杂媒虂斫鉀Q的話,那就不叫研究,頂多叫高中生生實(shí)驗(yàn)。 |

| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 北科281學(xué)碩材料求調(diào)劑 +6 | tcxiaoxx 2026-03-20 | 6/300 |
|
|---|---|---|---|---|
|
[考研] 一志愿 西北大學(xué) ,070300化學(xué)學(xué)碩,總分287,雙非一本,求調(diào)劑。 +3 | 晨昏線與星海 2026-03-20 | 3/150 |
|
|
[考研] 319求調(diào)劑 +4 | 小力氣珂珂 2026-03-20 | 4/200 |
|
|
[考研] 298求調(diào)劑一志愿211 +3 | 上岸6666@ 2026-03-20 | 3/150 |
|
|
[考研] 305分求調(diào)劑(食品工程) +4 | Sxy112 2026-03-21 | 6/300 |
|
|
[考研] 269專碩求調(diào)劑 +6 | 金恩貝 2026-03-21 | 6/300 |
|
|
[考研]
|
酥酥魚.. 2026-03-21 | 3/150 |
|
|
[考研] 0805 316求調(diào)劑 +3 | 大雪深藏 2026-03-18 | 3/150 |
|
|
[考研] 求助 +5 | 夢里的無言 2026-03-21 | 6/300 |
|
|
[考研] 085601調(diào)劑 358分 +3 | zzzzggh 2026-03-20 | 4/200 |
|
|
[考研] 求調(diào)劑 +6 | Mqqqqqq 2026-03-19 | 6/300 |
|
|
[考研] 316求調(diào)劑 +6 | 梁茜雯 2026-03-19 | 6/300 |
|
|
[考研] 化學(xué)求調(diào)劑 +4 | 臨澤境llllll 2026-03-17 | 5/250 |
|
|
[考研] 08工學(xué)調(diào)劑 +5 | 用戶573181 2026-03-20 | 5/250 |
|
|
[考研] 招收調(diào)劑碩士 +4 | lidianxing 2026-03-19 | 12/600 |
|
|
[考研] 求調(diào)劑 +3 | 暗涌afhb 2026-03-16 | 3/150 |
|
|
[考研] 301求調(diào)劑 +4 | A_JiXing 2026-03-16 | 4/200 |
|
|
[考研] 085601求調(diào)劑 +4 | Du.11 2026-03-16 | 4/200 |
|
|
[考研] 275求調(diào)劑 +4 | 太陽花天天開心 2026-03-16 | 4/200 |
|
|
[考研] 考研調(diào)劑 +3 | 淇ya_~ 2026-03-17 | 5/250 |
|