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huojinlong9287銀蟲 (小有名氣)
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[求助]
求助中文翻譯為英文一段話
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| ***是世界上第一個培育成功的大型哺乳動物近交系,在開展異種器官移植、疾病動物模型、轉基因動物、基因修飾、基因敲除及功能基因研究等方面具有巨大的應用價值。本研究擬針對***異種器官移植過程中與免疫排斥反應相關的新基因進行研究,通過采用數(shù)字基因表達譜方法進行高通量篩選基因,illumina測序,對測序結果評估,基因注釋,EST比對,篩選并初步確定與免疫排斥反應相關的新基因。采用real-time PCR和Northern-blotting檢測基因的表達水平,通過表達水平與免疫排斥反應的相關性分析,確定與排斥相關的關鍵功能基因,并進行多組織表達譜分析、功能預測和鑒定。為揭示異種移植中免疫排斥反應的相關研究奠定基礎,也可為下一步進行基因修飾和基因敲除確定重要的目標靶基因,最終為提高***在異種器官移植領域中的成功率提供科學依據。 |
新蟲 (著名寫手)
| *** is the world's first successful cultivation of a large mammal inbred,playing a important role in carrying out xenotransplantation, animal models of disease, transgenic animals, genetically modified, gene knockout and functional genomics research .This research focuses on new genes related to immune rejection during *** xenotransplantation Process , through the use of digital gene expression profiling method ,the high-throughput screening gene and illumina sequencing were carried out, the sequencing results was evaluated, gene annotation, EST comparison, the new gene associated with immune rejection was screened and determined preliminary. Expression of Gene was detected by the means of real-time PCR and Northern-blotting,the key gene associated with immune rejection was determined through correlation analysis of expression level and immune rejection,and multi-tissue expression pattern analysis, function prediction and identification were carried out.It is not only lay the foundation for revealing xenograft rejection in immune research,but also for identifying further important target gene in conduct of genetic modification and gene knockout ,finally it provides a scientific basis for increase of the success rate in the field of xenotransplantation. |

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