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huojinlong9287銀蟲(chóng) (小有名氣)
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[求助]
求助中文翻譯為英文一段話
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| ***是世界上第一個(gè)培育成功的大型哺乳動(dòng)物近交系，在開(kāi)展異種器官移植、疾病動(dòng)物模型、轉(zhuǎn)基因動(dòng)物、基因修飾、基因敲除及功能基因研究等方面具有巨大的應(yīng)用價(jià)值。本研究擬針對(duì)***異種器官移植過(guò)程中與免疫排斥反應(yīng)相關(guān)的新基因進(jìn)行研究，通過(guò)采用數(shù)字基因表達(dá)譜方法進(jìn)行高通量篩選基因，illumina測(cè)序，對(duì)測(cè)序結(jié)果評(píng)估，基因注釋?zhuān)珽ST比對(duì)，篩選并初步確定與免疫排斥反應(yīng)相關(guān)的新基因。采用real-time PCR和Northern-blotting檢測(cè)基因的表達(dá)水平，通過(guò)表達(dá)水平與免疫排斥反應(yīng)的相關(guān)性分析，確定與排斥相關(guān)的關(guān)鍵功能基因，并進(jìn)行多組織表達(dá)譜分析、功能預(yù)測(cè)和鑒定。為揭示異種移植中免疫排斥反應(yīng)的相關(guān)研究奠定基礎(chǔ)，也可為下一步進(jìn)行基因修飾和基因敲除確定重要的目標(biāo)靶基因，最終為提高***在異種器官移植領(lǐng)域中的成功率提供科學(xué)依據(jù)。 |
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yerufei_2010
新蟲(chóng) (著名寫(xiě)手)
- 翻譯EPI: 27
- 應(yīng)助: 0 (幼兒園)
- 金幣: 1.2
- 散金: 2528
- 紅花: 8
- 帖子: 1802
- 在線: 163.4小時(shí)
- 蟲(chóng)號(hào): 1181021
- 注冊(cè): 2010-12-31
- 性別: GG
- 專(zhuān)業(yè): 金屬有機(jī)化學(xué)
【答案】應(yīng)助回帖
★
愛(ài)與雨下(金幣+1): ��！ 2011-09-06 20:26:13
huojinlong9287(金幣+200, 翻譯EPI+1): 2011-09-07 21:54:02
愛(ài)與雨下(金幣+1): ��！ 2011-09-06 20:26:13
huojinlong9287(金幣+200, 翻譯EPI+1): 2011-09-07 21:54:02
| *** is the world's first successful cultivation of a large mammal inbred,playing a important role in carrying out xenotransplantation, animal models of disease, transgenic animals, genetically modified, gene knockout and functional genomics research .This research focuses on new genes related to immune rejection during *** xenotransplantation Process , through the use of digital gene expression profiling method ，the high-throughput screening gene and illumina sequencing were carried out, the sequencing results was evaluated, gene annotation, EST comparison, the new gene associated with immune rejection was screened and determined preliminary. Expression of Gene was detected by the means of real-time PCR and Northern-blotting,the key gene associated with immune rejection was determined through correlation analysis of expression level and immune rejection，and multi-tissue expression pattern analysis, function prediction and identification were carried out.It is not only lay the foundation for revealing xenograft rejection in immune research，but also for identifying further important target gene in conduct of genetic modification and gene knockout ,finally it provides a scientific basis for increase of the success rate in the field of xenotransplantation. |
2樓2011-09-06 20:08:11
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