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wxy20052002木蟲 (著名寫手)
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[求助]
兼并引物的表示方法
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下列兼并引物的表示方法讀不懂。如何用兼并引物表的形式表示出來?謝謝! F 5’-GC/T/C/GCAT/CCCA/T/CAAG/AG/CT/CG/C/TGAT/CCC-3’ R 5’-TCA/GGTG/AATA/G/T/GCG/AAAG/ATCAT-3’ |
金蟲 (初入文壇)
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Field D3 Code Deoxyadenine A Deoxycytosine C Deoxyguanidine G Deoxythymidine T Deoxyuracil U Deoxyinosine I Phosphorothioate-A F Phosphorothioate-C O Phosphorothioate-G E Phosphorothioate-T Z A+C+G V A+T+G D T+C+G B Field D3 Code A+T+C H A+T W C+G S T+G K A+C M C+T Y A+G R A+G+C+T N |
金蟲 (正式寫手)

榮譽(yù)版主 (知名作家)
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轉(zhuǎn)一個(gè): IUPAC ambiguity codes are single-letter codes for nucleotides and amino acids established by the International Union of Pure and Applied Chemistry. This nomenclature is used for specifying Pattern Search parameters. Nucleotides (Cornish-Bowden, 1985): Symbol Meaning G G A A T T or U C C U U or T R G or A Y T, U or C M A or C K G, T or U S G or C W A, T or U H A, C, T or U B G, T, U or C V G, C or A D G, A, T or U N G, A, T, U or C Amino Acids: 1-Letter Symbol 3-Letter Symbol Meaning A Ala Alanine R Arg Arginine N Asn Asparagine D Asp Aspartic acid C Cys Cysteine Q Gln Glutamine E Glu Glutamic acid G Gly Glycine H His Histidine I Ile Isoleucine L Leu Leucine K Lys Lysine M Met Methionine F Phe Phenylalanine P Pro Proline S Ser Serine T Thr Threonine W Trp Tryptophan Y Tyr Tyrosine V Val Valine B Asx Asparagine or Aspartic acid Z Glx Glutamine or Glutamic acid X ??? Unknown Cornish-Bowden, A. (1985). Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984. Nuc. Acids Res. 13:3021-3030. |
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