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| 本帖產(chǎn)生 1 個(gè) MolEPI ,點(diǎn)擊這里進(jìn)行查看 | |||
[求助]
求助小鼠抗體可變區(qū)基因序列提交問(wèn)題
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前段時(shí)間克隆了幾個(gè)小鼠抗體可變區(qū)的基因序列,并且上傳到NCBI,最近收到來(lái)信說(shuō)其中一個(gè)序列可能存在移碼,讓我確認(rèn)序列是否正確。如果正確,讓我提供相應(yīng)證據(jù)證明。我確定自己上傳的序列是正確的測(cè)序結(jié)果,不知該如何提供證據(jù),請(qǐng)有相關(guān)經(jīng)驗(yàn)人士幫忙指導(dǎo)一下。信件原文如下: We are currently processing your GenBank records JN109176-JN109189 and have come across an issue that requires your attention. Upon doing a BLAST similarity search of your sequences, we noticed that there may be a reading frame shift in the following translation relative to similar proteins: JN109185 around nuc 287-288 If the nucleotide sequence is incorrect, send us the corrected sequence, and we will replace the current sequence in your submission. If the sequence is correct, please let us know if you have any evidence that this frame shifted protein is translated. |
【分子生物】EPI帖 |
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鐵桿木蟲 (正式寫手)
| atttccagcttggtccccgagccgaacgtgaaacacagtgctgacagaaatacattgcaatatcatcctcctccacaggatggatgttgaggctgaagtctgtcccagacccactgccactaaacctggcagggaccccagattctacgttggatgcagcatagatgaggagtttgggtggctgtcctggtttctgttggtaccactgcattaaacttgtgccataatattcaacactttcactggctctgcaggagatggtggctctctgccctagagacacagccaaagaagctggagactggg |
銀蟲 (小有名氣)
金蟲 (小有名氣)

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