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phitong木蟲 (小有名氣)
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[求助]
土壤碳庫(kù)測(cè)定
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現(xiàn)在小蟲做實(shí)驗(yàn)，欲采用Rovira and Vallejo(2002)的方法來(lái)分析土壤活性和惰性有機(jī)碳庫(kù)。 Rovira and Vallejo(2002)文章簡(jiǎn)述如下：Briefly, 20 mL of 5NH2SO4 was added to 500 mg soil, and the sample was hydro- lyzed for 30 min at 105 C in sealed Pyrex tubes, after which the hydrolysate was recovered by centrifugation and decantation. The residue was washed with 20 mL of de-ionized water and the washing added to the hydrolysate. This hydrolysate was taken as labile pool I (LPI) and analyzed for labile pool I carbon (LPI-C) and nitrogen (LPI-N). The remaining residue was hydrolyzed with 2 mL of 26 N H2SO4 overnight at room temperature under continuous shaking. The concentration of the acid was then brought down to 2 N by dilution with de-ionized water and the sample was hydro- lyzed for 3 h at 105 Cwith occasional shaking. The hydrolysatewas recovered in the same manner as for the LPI. This second hydro- lysatewas taken as labile pool II (LPII) and analyzed for labile pool II carbon (LPII – C) and nitrogen (LPII – N). 問(wèn)題如下：1.離心速度多大合適？ 2.稱量土，加入2.5 M H2SO4溶液這一步可以直接放在離心管中高溫水解嗎？不可以的話，該用什么容器，之后如何無(wú)損失轉(zhuǎn)移？ |
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phitong
木蟲 (小有名氣)
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9樓2012-01-06 10:31:05
phitong
木蟲 (小有名氣)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 2370.6
- 散金: 88
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- 帖子: 287
- 在線: 145.4小時(shí)
- 蟲號(hào): 776643
- 注冊(cè): 2009-05-22
- 專業(yè): 全球變化生態(tài)學(xué)
2樓2012-01-05 09:17:52
感謝參與，應(yīng)助指數(shù) +1
zongyu_1986(應(yīng)助指數(shù)-1): 無(wú)效應(yīng)助，扣除應(yīng)助指數(shù) 2012-01-06 12:29:15
zongyu_1986(應(yīng)助指數(shù)-1): 無(wú)效應(yīng)助，扣除應(yīng)助指數(shù) 2012-01-06 12:29:15
3樓2012-01-05 19:50:01
4樓2012-01-05 19:50:17
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