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北京石油化工學院2026年研究生招生接收調(diào)劑公告

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炮打總統(tǒng)府

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A 1 L Erlenmeyer flask was equipped with an oxygen electrode connected to a recorder. The solution for the SOUR test for ammonium oxidizers composed of 100 mg N/L ammonium and other minerals without any organic compound or nitrite. The solution for nitrite oxidizers composed of 100 mg N/L nitrite and other minerals without organic compound or ammonium. A control test contained only minerals. A mixed liquor sample or a detached-biofilm suspension was saturated with oxygen by shaking and transferred to the flask.The initial biomass concentration in the batch reactors was in the range of 700–1000 mg VSS/L for suspended growth cells and 150–300 mg VSS/L for detached biofilm cells. The flask was carefully closed without leaving air bubbles. For the attached cell experiment, the detachment of the biofilm from the surface of the media collected from the reactor was performed using a vortex (MO Bio Vortex) for 5 min at the maximum speed and by adding polyox-yethylene lauryl ether to aid in removing cells from surfaces and promoting
their lyses . This procedure was repeated three times. The degree of detachment was monitored to determine the suitability of the method by measuring microbial activity remaining in the medium by adenosine triphosphate (ATP) and no activity was observed in the medium (data not shown).The cell suspension was stirred with a magnetic stirrer at 150 rpm, 30 8C and pH 8, respectively. The SOUR (mg O
2/g VSS d) of each sample was equal to the slope of DO depletion versus time divided by the VSS in the flask.

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【答案】應助回帖

炮打總統(tǒng)府(金幣+20, 翻譯EPI+1): ★★★很有幫助 2012-02-17 17:21:42

如下，僅供參考。polyox-yethylene lauryl ether 試劑名稱未能譯出。。。

在一只1升的錐形瓶中裝入連有記錄器的氧電極。在SOUR測試中，用于測試氨氧化細菌的溶液含100 mg N/L 的氨和其他礦物質，且不含任何有機成分或亞硝酸鹽。用于測試亞硝酸鹽氧化細菌的溶液中含100 mg N/L 亞硝酸鹽其他礦物質，且不含任何有機成分或氨。對照試驗（的溶液中）只含礦物質。通過搖晃使混合試液或分離生物膜的懸浮液中的溶解氧達到飽和，然后將其轉移到該錐形瓶中。序批式反應器中含有的初始生物量濃度為：700–1000 mg VSS/L 懸浮污泥生物量和 150–300 mg VSS/L 的分離生物膜生物量。小心地將錐形瓶封閉起來，確保瓶中沒有殘留氣泡。
在附帶的細胞試驗中，將從反應器中收集的生物膜媒質放入漩渦（分離器）來分離表面的生物膜。分離轉速設為最大，分離時間為5分鐘。并加入polyox-yethylene lauryl ether 來幫助細胞從表面分離并促進細胞溶解。該過程重復三次。為了檢測生物膜分離的程度和衡量該方法的適用性，本實驗采用adenosine triphosphate (ATP)方法檢測媒質上剩余的生物活性。檢測結果顯示媒質不再具有生物活性（數(shù)據(jù)未顯示）。細胞懸浮液用磁力攪拌器在150 rpm的速度下攪拌，液體溫度30.8C，pH 值為8。各種試液中的SOUR等于溶解氧消耗隨時間變化（曲線）的斜率除以錐形瓶中的VSS。

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