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綠茶珍金蟲 (小有名氣)
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[求助]
膜蛋白提取試劑盒問題
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| 最近要做真菌膜蛋白的分離提取~我想問一下膜蛋白提取試劑盒能行嗎？ 試劑盒原理是什么丫，一次提取的量有多少，可以提取多少次？ 謝謝各位前輩了 |
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xiaoyu0127
銅蟲 (小有名氣)
- 應(yīng)助: 0 (幼兒園)
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- 性別: MM
- 專業(yè): 生物化學(xué)
3樓2013-06-28 10:18:04
xhjggl
木蟲 (正式寫手)
- BioEPI: 1
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【答案】應(yīng)助回帖
感謝參與，應(yīng)助指數(shù) +1
綠茶珍(金幣+5): 2012-03-01 08:36:00
綠茶珍(金幣+5): 2012-03-01 08:36:00
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FOCUS™ Global Fractionation 膜蛋白分級分離和胞質(zhì)蛋白提取試劑盒 Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins. Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling. where one prep is:100mg mammalian tissue or 50µl wet animal cell pellet or 50µl wet yeast pellet or 50µl wet bacteria pellet or 250mg plant tissue. Store：-15～-20℃ Features: Fractionate integral, peripheral and lipid raft associated proteins.Fractionation of complex proteomes into multiple, specific fractions.applification for Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources.Fractionation of membrane proteins from lipid rafts.Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.Suitable for proteomics and cell signaling studies. 編號 包裝 價格 產(chǎn)地 786-018 50 Assays 2406 G Bios |
2樓2012-02-29 22:43:29
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