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heihei3910銅蟲 (小有名氣)
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[求助]
重金求助基因克隆與功能驗證方向 中文摘要350字左右翻譯成英語 急！ 急！ 急！在線等
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針對赤霉病導致小麥嚴重減產(chǎn)，而化學防治和抗病新品種均無法抑制赤霉病的危害問題，以本實驗室發(fā)現(xiàn)的放線菌JK-1聚酮化合物能高效抑制赤霉病菌為契機，采用簡并引物制備探針、構(gòu)建基因組BAC文庫獲取PKS基因全長，并進行PKS基因的大腸桿菌表達及農(nóng)桿菌介導的小麥轉(zhuǎn)化表達，研究PKS基因在小麥中成功轉(zhuǎn)化并表達的技術(shù)難題，探討PKS基因在小麥的抗病作用分子機理，闡明PKS基因在小麥赤霉病防控方面的重大作用。項目預期將建立PKS基因轉(zhuǎn)化小麥并成功表達的技術(shù)體系，揭示PKS基因抗赤霉病的分子機理。項目的研究結(jié)果對于獲得比傳統(tǒng)育種及轉(zhuǎn)基因抗病小麥品種更穩(wěn)定而持久的抗赤霉病小麥品種具有重要意義。項目的實施可以為PKS基因在植物上成功表達并表現(xiàn)出很好的抗病性提供科學依據(jù)。 [ Last edited by heihei3910 on 2012-3-5 at 22:11 ] |
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heihei3910
銅蟲 (小有名氣)
- 應助: 0 (幼兒園)
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- 帖子: 64
- 在線: 30.8小時
- 蟲號: 761765
- 注冊: 2009-05-02
- 性別: MM
- 專業(yè): 生物信息學
4樓2012-03-06 20:55:53
hbx924
新蟲 (小有名氣)
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【答案】應助回帖
★ ★ ★
Mally89(金幣-3, 違規(guī)存檔): 機器翻譯，鑒于新蟲，扣除3個BB以示警告！~請蟲友應助前閱讀本版《應助者必讀》帖子。多謝理解合作！~歡迎常來！~ 2012-03-06 16:40:01
Mally89(金幣-3, 違規(guī)存檔): 機器翻譯，鑒于新蟲，扣除3個BB以示警告！~請蟲友應助前閱讀本版《應助者必讀》帖子。多謝理解合作！~歡迎常來！~ 2012-03-06 16:40:01
| So a lead to bad time for wheat, and chemical control and disease-resistant varieties are so couldn't restrain the harm to the lab found ray JK-1 sulfo-benzophenone compounds can inhibit together efficient fusarium graminearum as an opportunity, USES the Jane and primer preparation probe, building library PKS genes for genome BAC span, and the PKS gene expression and e. coli agrobacterium mediated wheat into express, the PKS genes in wheat successful conversion and expression in the technical problems, this paper discusses the genes in wheat PKS diseases of the molecular mechanism function, expounds the PKS genes in wheat genotypes of prevention and control of the major role. The project is expected to will build PKS genetic transformation and success of wheat express technical system, and reveals PKS genes on molecular mechanism of heavy resistance. Project results for compared with the traditional breeding genetically modified disease-resistant wheat varieties and more stable and enduring resistance varieties of wheat genotypes has important significance. The implementation of the project for the PKS genes in plants express and show successful on very good resistance to provide the scientific basis. |
2樓2012-03-06 13:07:53
weichin
鐵桿木蟲 (職業(yè)作家)
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【答案】應助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
Mally89(金幣+1): 感謝應助！~歡迎常來！~吼吼！~ 2012-03-06 16:40:16
heihei3910(金幣+100, 翻譯EPI+1): ★★★很有幫助 謝謝你，雖然有不準確的地方，但還是非常感謝你了�。�！ 2012-03-06 20:58:33
Mally89(金幣+1): 感謝應助！~歡迎常來！~吼吼！~ 2012-03-06 16:40:16
heihei3910(金幣+100, 翻譯EPI+1): ★★★很有幫助 謝謝你，雖然有不準確的地方，但還是非常感謝你了�。�！ 2012-03-06 20:58:33
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供參考： Gibberellic disease cause serious cuts in wheat production, while neither chemical control and prevention nor disease-resistant varieties succeeded in inhibiting it. By taking advantage of the actinomycete JK-1 polyketide found in our laboratory which has proved to be highly effective in inhibiting Fusarium graminearum, the present study, through preparation of probes using degenerate primer and obtaining of full-length PKS genes by constructing a genomic BAC library, completed the PKS gene expression in Escherichia coli and Agrobacterium-mediated transient expression of wheat, solved the technical difficulties in the transformation and expression of PKS genes in wheat, addressed the molecular mechanism of PKS gene’s disease-resistant function, and clarified the significant role PKS genes played in preventing and controlling wheat scab. The project is expected to establish a set of techniques for the transformation and expression of PKS genes in wheat, revealing the molecular mechanism of the PKS gene’s resistance to wheat scab. The research findings of the project shall shed light on obtaining wheat varieties with more stable and lasting resistance to wheat scab, compared with traditional breeding and transgenic disease-resistant wheat varieties. Implementation of the project scientific could provide evidence for the successful expression of PKS genes in plants and their strong resistance to diseases. |
3樓2012-03-06 14:00:12
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