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[求助]
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| 脂氧酶（LOX）為活性中心含鐵的金屬酶。本文將以大豆LOX為模板，研究外源金屬離子對LOX活性中心的影響及其對酶活的調(diào)控，驗證和完善我們提出的外源金屬離子與LOX活性中心離子存在離子交換的假說，研究LOX催化過程中的電子傳遞/離子交換機制。利用Irving-Williams序列實現(xiàn)一些外源過度金屬離子與LOX中鐵的交換，研究其它金屬離子對LOX的活性或酶促反應(yīng)的影響；采用自制新型指示劑結(jié)合光譜技術(shù)在一定范圍內(nèi)定性定量地研究LOX活性的重構(gòu)對其結(jié)構(gòu)、活性及催化動力學(xué)的影響；實現(xiàn)對LOX的金屬修飾并調(diào)控其催化動力學(xué)。本文是金屬酶化工的研究和應(yīng)用中亟需解決的共性問題，是對酶化工、天然酶修飾及金屬離子生物效應(yīng)等的應(yīng)用基礎(chǔ)研究；對了解原生態(tài)金屬酶活性中心金屬離子的作用機制、內(nèi)外源金屬離子間的相互作用及其活性調(diào)控具有普遍理論意義，也可為研究其它酶促反應(yīng)中金屬離子的作用機制提供方法上的參考。 |
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
愛與雨下: 金幣+2 2012-03-15 10:28:24
sltmac: 金幣+20 2012-03-20 12:29:18
愛與雨下: 金幣+2 2012-03-15 10:28:24
sltmac: 金幣+20 2012-03-20 12:29:18
|
LOX is a metalloenzyme with Fe in its active site. In this article, We have investigated the effects of exogenetic metal ions on the active site and activities of LOX from soybean.The results validated and improved our hypothesis that there are exchanges between exogenetic metal ions and ions in LOX active site.(或者是The results validated and improved our hypothesis that exogenetic metal ions can exchange with ions in LOX active site.)We also researched the mechanism of electron transfer during catalytic process of LOX. 這是到“脂氧酶（LOX）為活性中心含鐵的金屬酶。本文將以大豆LOX為模板，研究外源金屬離子對LOX活性中心的影響及其對酶活的調(diào)控，驗證和完善我們提出的外源金屬離子與LOX活性中心離子存在離子交換的假說，研究LOX催化過程中的電子傳遞/離子交換機制” 后面的中文有點亂，翻不下去了  |
4樓2012-03-14 14:27:37
cheungsober
木蟲 (正式寫手)
- 翻譯EPI: 9
- 應(yīng)助: 2 (幼兒園)
- 金幣: 2382.4
- 散金: 3
- 紅花: 1
- 帖子: 498
- 在線: 111.1小時
- 蟲號: 217544
- 注冊: 2006-03-12
- 專業(yè): 生物化學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
愛與雨下: 金幣+3 2012-03-15 10:28:33
sltmac: 金幣+30, 翻譯EPI+1 2012-03-20 12:29:11
愛與雨下: 金幣+3 2012-03-15 10:28:33
sltmac: 金幣+30, 翻譯EPI+1 2012-03-20 12:29:11
|
水平有限，僅供參考。 Effect of enzyme (LOX) is enzyme active site iron metal. The LOX of soybean being a template, we research on exogenous effects of metal ions on the activity of LOX Center and its regulation of enzyme activity, validation and improvement of metal ion and exogenous LOX activity of our center of ion-ion exchange hypothesis study on catalytic process of electron transfer in LOX/ion exchange mechanism. Using Irving-Williams sequence implementation some outside source excessive metal ion and LOX in the iron of Exchange, research other metal ion on LOX of activity or enzyme promoting reaction of effect; used homemade newly indicates agent combination spectrum technology in must range within qualitative quantitative to research LOX activity of heavy frame on its structure, and activity and the catalytic dynamics of effect; implementation on LOX of metal modified and regulation its catalytic dynamics. This is metal enzyme chemical of research and application in the needed solution of common problem, is on enzyme chemical, and natural enzyme modified and the metal ion bio-effect, of application foundation research; for understanding original ecological metal enzyme activity center metal ion of role mechanism, and inside and outside source metal ion between of mutual role and activity regulation has general theory significance, also can for research other enzyme promoting reaction in the metal ion of role mechanism provides method Shang of reference. |
2樓2012-03-13 23:32:05
【答案】應(yīng)助回帖
|
LOL is a metalloenzyme with Fe in its active site. In this article, We have investigated the effects of exogenetic metal ions on the active site and activities of LOL from soybean.The results validated and improved our hypothesis that there are exchanges between exogenetic metal ions and ions in LOL active site.(或者是The results validated and improved our hypothesis that exogenetic metal ions can exchange with ions in LOL active site.) 沒翻完，先占著，目前繼續(xù)翻。原文中有大段的長句，寫成英文時，應(yīng)該根據(jù)意思分成短句，太長了意思又混亂是不行的。希望對lz有幫助。 |
3樓2012-03-14 14:19:45
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