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[求助]
關于熒光傳感薄膜的幾個問題
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現(xiàn)在我正在做關于熒光傳感薄膜,遇到了幾個問題,想請高人指點: 1. 在測膜的熒光的時候,是否要重點考慮膜表面的漫反射情況? 2. 在第一個問題的基礎上,幾張膜經過不同的處理之后,測得的熒光強度來熒光強度是否可以比較? 3. 如果熒光分子是靠靜電作用發(fā)生的熒光淬滅,那么,該過程的速度快嗎? 謝謝! |

專家顧問 (著名寫手)
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專家經驗: +19 |
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(1) If fluorophores are embedded inside the film, you should consider the possbile loss of light owing the scattering/reflection/absorption of film materials. (2) It depends on how you treat with these films. If the optical properties do not change significantly, I think you can make a rough comparison. (3) It depends on how fast these quenchers can diffuse into the film for interaction. The charge, size of 'quenchers' can all affect the result. |
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Thank you! In some studies, fluorophores are indeed embedded inside the film whose thickness is ca. 10 μm, they used this film as sensors to detect pH via measuring the change of fluorescence intensity. And during the process, they fix position of film making sure that lase rpulse irradiates at the same position. My question is "whether this means can be used to accurately detect pH as a sendor or not". |

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