| 3 | 1/1 | 返回列表 |
| 查看: 428 | 回復: 2 | ||||
[求助]
關于熒光傳感薄膜的幾個問題
|
|
現在我正在做關于熒光傳感薄膜,遇到了幾個問題,想請高人指點: 1. 在測膜的熒光的時候,是否要重點考慮膜表面的漫反射情況? 2. 在第一個問題的基礎上,幾張膜經過不同的處理之后,測得的熒光強度來熒光強度是否可以比較? 3. 如果熒光分子是靠靜電作用發(fā)生的熒光淬滅,那么,該過程的速度快嗎? 謝謝! |

專家顧問 (著名寫手)
![]() |
專家經驗: +19 |
|
(1) If fluorophores are embedded inside the film, you should consider the possbile loss of light owing the scattering/reflection/absorption of film materials. (2) It depends on how you treat with these films. If the optical properties do not change significantly, I think you can make a rough comparison. (3) It depends on how fast these quenchers can diffuse into the film for interaction. The charge, size of 'quenchers' can all affect the result. |
|
Thank you! In some studies, fluorophores are indeed embedded inside the film whose thickness is ca. 10 μm, they used this film as sensors to detect pH via measuring the change of fluorescence intensity. And during the process, they fix position of film making sure that lase rpulse irradiates at the same position. My question is "whether this means can be used to accurately detect pH as a sendor or not". |

| 3 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 11408總分309,一志愿東南大學求調劑,不挑專業(yè) +5 | 天賦帶到THU 2026-03-29 | 6/300 |
|
|---|---|---|---|---|
|
[考研] 297求調劑 +3 | 田洪有 2026-03-30 | 3/150 |
|
|
[考研] 085600 295分求調劑 +7 | W55j 2026-03-30 | 9/450 |
|
|
[考研] 332求調劑 +14 | 032500 2026-03-25 | 14/700 |
|
|
[考研] 085600 286分 材料求調劑 +11 | 麻辣魷魚 2026-03-27 | 12/600 |
|
|
[考研] 309求調劑 +15 | 誰不是少年 2026-03-29 | 15/750 |
|
|
[考研] 295材料工程專碩求調劑 +10 | 1428151015 2026-03-27 | 10/500 |
|
|
[考研] 310求調劑 +10 | 爭取九點睡 2026-03-30 | 10/500 |
|
|
[考研] 0703 化學 求調劑,一志愿山東大學 342 分 +7 | Shern—- 2026-03-28 | 7/350 |
|
|
[考研] 材料科學與工程調劑 +8 | 深V宿舍吧 2026-03-30 | 8/400 |
|
|
[考研] 化學308分調劑 +7 | 你好明天你好 2026-03-30 | 8/400 |
|
|
[考研] 086000生物與醫(yī)藥調劑 +5 | Feisty。 2026-03-28 | 9/450 |
|
|
[考研] 332求92調劑 +8 | 蕉蕉123 2026-03-28 | 8/400 |
|
|
[考研] 0703化學調劑,求導師收 +9 | 天天好運來上岸?/a> 2026-03-24 | 10/500 |
|
|
[考研] 本科新能源科學與工程,一志愿華理能動285求調劑 +3 | AZMK 2026-03-27 | 5/250 |
|
|
[考研] 283求調劑 +7 | A child 2026-03-28 | 7/350 |
|
|
[考研] 316求調劑 +5 | Pigcasso 2026-03-24 | 5/250 |
|
|
[考研] 求調劑 +6 | 林之夕 2026-03-24 | 6/300 |
|
|
[考研] 303求調劑 +6 | 藍山月 2026-03-25 | 6/300 |
|
|
[考研] 生物學學碩求調劑 +7 | 小羊睡著了? 2026-03-23 | 10/500 |
|