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酸乳娃娃新蟲 (小有名氣)
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[求助]
Zeta電勢測定pH問題
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| 大家好,測定納米粒子 zeta電勢的時候,我想測定不同pH值下的電勢。這些不同pH值的溶液是怎么準備呢?我想測定pH2到10之間的電勢變化。多謝! |
專家顧問 (著名寫手)
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專家經(jīng)驗: +19 |
配制對應(yīng)pH范圍的緩沖溶液,然后將你的納米粒子分散在該緩沖溶液即可。附件為常見緩沖溶液的配制方法,可以參考。 |
專家顧問 (知名作家)
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專家經(jīng)驗: +181 |
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在不同pH下zeta很難測,意思是與樣品制備方式有關(guān),不同人做的話很難有重復性。pH是一方面,調(diào)pH過程中把離子強度也改變了。 但應(yīng)該注意一下幾點: 1. Samples should be prepared in a low ionic strength medium. 10 mM NaCl is recommended. 2. As a result, the zeta potential must be measured at different concentrations. 3. Rapid addition of strong acid or base may compromise the sample’s integrity and result in a polydispersed sample. In this case, the zeta potential distribution may contain multiple peaks arising from the presence of differently charged species.It is recommended to titrate with 0.1 M HCl or 0.1 M NaOH in a drop-wise (~1–3 mL) manner and monitor the pH throughout. 4. Zeta potential is dependent on pH and therefore the sample pH should be measured before and after the zeta potential readings. |
專家顧問 (知名作家)
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專家經(jīng)驗: +181 |
新蟲 (小有名氣)
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