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[求助]
誰(shuí)有頭孢美唑內(nèi)酯(cefmetazole lactone)的結(jié)構(gòu)式
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誰(shuí)有頭孢美唑內(nèi)酯(cefmetazole lactone)的結(jié)構(gòu)式? 頭孢美唑有關(guān)物質(zhì)做液質(zhì)大家都用什么液相條件啊? |

銅蟲(chóng) (初入文壇)
銀蟲(chóng) (小有名氣)




版主 (文壇精英)
老痞
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JP16液相方法 Detector: An ultraviolet absorption photometer (wave- length: 214 nm). Column: A stainless steel column 4.6 mm in inside diame- ter and 25 cm in length, packed with octadecylsilanized silica gel for liquid chromatography (5 mm in particle diameter). Column temperature: A constant temperature of about 25℃ Mobile phase: Dissolve 5.75 g of ammonium dihydrogen- phosphate in 700 mL of water, add 280 mL of methanol, 20 mL of tetrahydrofuran and 3.2 mL of 40% tetrabutylammo- nium hydroxide TS, and adjust to pH 4.5 with phosphoric acid. Flow rate: Adjust the flow rate so that the retention time of cefmetazole is about 8 minutes. System suitability System performance: When the procedure is run with 10 mL of the standard solution under the above operating con- ditions, cefmetazole and the internal standard are eluted in this order with the resolution between these peaks being not less than 10. System repeatability: When the test is repeated 5 times with 10 mL of the standard solution under the above operat- ing conditions, the relative standard deviation of the ratios of the peak area of cefmetazole to that of the internal stand- ard is not more than 2.0%. |

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