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fbwg金蟲 (小有名氣)
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[求助]
求助1段話的英譯漢審校,關(guān)于臨床微生物方面的
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The most frequent technical pitfall encountered in microbiology laboratories performing bacteri¬cidal testing is the use of stationary-phase cultures. The rate of loss of viability of bacterial cells following exposure to most antibiotics with an especially marked effect on cell-wall-active agents is a direct function of bacterial growth prior to the addition of the agent.17,45 Aminoglycosides and quinolones, however, are much less affected by the use of slowly growing cultures.45 The use of stationary phase cultures, such as recently inoculated cultures growing for periods >8 hours, will include an increased number of dormant cells which are not as susceptible to the agent and therefore cause diminished killing rates. 微生物學(xué)實(shí)驗(yàn)室進(jìn)行殺菌實(shí)驗(yàn)最常遇到的技術(shù)困難是利用靜止期培養(yǎng)物。暴露于多數(shù)抗生素后細(xì)菌細(xì)胞活力損失率對(duì)破細(xì)胞壁藥物有特殊影響是在加入藥物之前細(xì)菌生長(zhǎng)的直接功能17,45。然而,通過使用生長(zhǎng)慢的培養(yǎng)物,氨基糖苷類和喹諾酮類藥物受到的影響會(huì)小很多45。利用靜止期培養(yǎng)物,諸如利用近期接種的培養(yǎng)基生長(zhǎng)時(shí)間超過8小時(shí),將使休眠細(xì)胞數(shù)量增加,而休眠細(xì)胞對(duì)藥物并不敏感因此導(dǎo)致殺菌率降低。 |
木蟲 (正式寫手)
| 微生物學(xué)實(shí)驗(yàn)室進(jìn)行殺菌實(shí)驗(yàn)最常遇到的技術(shù)陷阱是利用靜止期培養(yǎng)物。暴露于多數(shù)抗生素——尤其是顯著影響破壞細(xì)胞壁試劑的抗生素——造成的細(xì)菌細(xì)胞活力損失率在加入破壞細(xì)胞壁試劑之前直接影響細(xì)菌生長(zhǎng)。然而,氨基糖苷類和喹諾酮類藥物對(duì)生長(zhǎng)緩慢的培養(yǎng)物影響很小。利用靜止期培養(yǎng)物,諸如新近接種的培養(yǎng)物生長(zhǎng)8小時(shí)以后,將增加對(duì)破壞細(xì)胞壁試劑不敏感的休眠期細(xì)胞數(shù)量,結(jié)果導(dǎo)致殺菌率降低。 |
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