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[求助]
FITC標(biāo)記蛋白的問題
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| 請問FITC標(biāo)記蛋白的過程中,使用DMSO溶解FITC,然后加入蛋白質(zhì)溶液,這里DMSO的加入會(huì)不會(huì)破壞蛋白質(zhì)的結(jié)構(gòu),導(dǎo)致蛋白質(zhì)失活?如果會(huì)破壞蛋白質(zhì)的構(gòu)象,那么應(yīng)該用什么溶解FITC??? |
細(xì)胞實(shí)驗(yàn) |
木蟲 (正式寫手)
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HOOK™ Dye Labeling Kit (FITC) 蛋白FITC標(biāo)記試劑盒 Description: FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. Features: Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15~-20℃ 編號 包裝 價(jià)格 產(chǎn)地 786-059 5 Reactions 2,709 GBios |
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專家顧問 (著名寫手)
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專家經(jīng)驗(yàn): +61 |
木蟲 (正式寫手)
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