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微生物方面幾句話
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| 下面幾句話比較難懂,請求各位幫忙。謝謝! The purified OmcS antibodies were tested for specificity using Western blot analysis (One-Step Western complete kit with tetramethyl benzidine [TMB]; GenScript) of total proteins prepared from cell lysates of the G. sulfurreducens wild type and the mutant strain in which the gene encoding OmcS had been deleted. Total proteins (10 g) were separated by 10% SDS-PAGE, blotted onto membranes using a semidry transfer cell (Bio-Rad), and incubated with the purified OmcS antibodies. Only one band with a molecular mass corresponding to that of the OmcS protein (i.e., 50 kDa) was detected with Western blot analysis in the wild-type cell lysate, whereas no bands were detected in the cell lysate |
至尊木蟲 (職業(yè)作家)
| 我們用特異性印跡(一步法西方完整套件四甲基聯(lián)苯胺 [TMB] ;)分析提純Omcs 的總蛋白細胞G蛋白質(zhì)組。在這些全蛋白中野生型及突變株的 OmcS 基因已被刪除。通過10% SDS-PAGE和Bio-Rad轉(zhuǎn)移,以及提純的OcmS抗體,我們分離了10g全蛋白.在野生類型的細胞中,只有一個與OmcS蛋白質(zhì)分子量相似的分子(例如,50KDa)可以用特異性印跡分析檢測,其它的則不能。 |

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