| 2 | 1/1 | 返回列表 |
| 查看: 535 | 回復(fù): 1 | |||
| 本帖產(chǎn)生 1 個 翻譯EPI ,點擊這里進行查看 | |||
[求助]
微生物方面幾句話
|
|||
| 下面幾句話比較難懂,請求各位幫忙。謝謝! The purified OmcS antibodies were tested for specificity using Western blot analysis (One-Step Western complete kit with tetramethyl benzidine [TMB]; GenScript) of total proteins prepared from cell lysates of the G. sulfurreducens wild type and the mutant strain in which the gene encoding OmcS had been deleted. Total proteins (10 g) were separated by 10% SDS-PAGE, blotted onto membranes using a semidry transfer cell (Bio-Rad), and incubated with the purified OmcS antibodies. Only one band with a molecular mass corresponding to that of the OmcS protein (i.e., 50 kDa) was detected with Western blot analysis in the wild-type cell lysate, whereas no bands were detected in the cell lysate |
至尊木蟲 (職業(yè)作家)
| 我們用特異性印跡(一步法西方完整套件四甲基聯(lián)苯胺 [TMB] ;)分析提純Omcs 的總蛋白細(xì)胞G蛋白質(zhì)組。在這些全蛋白中野生型及突變株的 OmcS 基因已被刪除。通過10% SDS-PAGE和Bio-Rad轉(zhuǎn)移,以及提純的OcmS抗體,我們分離了10g全蛋白.在野生類型的細(xì)胞中,只有一個與OmcS蛋白質(zhì)分子量相似的分子(例如,50KDa)可以用特異性印跡分析檢測,其它的則不能。 |

| 2 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 085601一志愿中山大學(xué)深圳材料工程330求調(diào)劑 +5 | pipiver 2026-03-30 | 5/250 |
|
|---|---|---|---|---|
|
[考研] 食品工程專碩一志愿中海洋309求調(diào)劑 +5 | 小張zxy張 2026-03-26 | 10/500 |
|
|
[考研] 22408 359分調(diào)劑 +4 | Qshers 2026-03-27 | 7/350 |
|
|
[考研]
|
鐘llll 2026-03-26 | 5/250 |
|
|
[考研] 材料學(xué)碩333求調(diào)劑 +14 | 北道巷 2026-03-24 | 14/700 |
|
|
[考研] 化工專碩求調(diào)劑 +5 | question挽風(fēng) 2026-03-24 | 5/250 |
|
|
[考研] 322求調(diào)劑:一志愿湖南大學(xué) 材料與化工(085600),已過六級。 +9 | XX小鄧 2026-03-29 | 9/450 |
|
|
[考研] 375求調(diào)劑 +6 | 雨夏整夜 2026-03-29 | 6/300 |
|
|
[考研] 321求調(diào)劑 +7 | 璞玉~~ 2026-03-25 | 8/400 |
|
|
[考研] 復(fù)試調(diào)劑 +3 | raojunqi0129 2026-03-28 | 3/150 |
|
|
[考研] 0856,材料與化工321分求調(diào)劑 +12 | 大饞小子 2026-03-27 | 13/650 |
|
|
[論文投稿] Journal of Mechanical Science and Technology +3 | Russ_ss 2026-03-25 | 5/250 |
|
|
[考研] 351求調(diào)劑 +4 | 麥克阿磊 2026-03-24 | 4/200 |
|
|
[考研] 總分322求生物學(xué)/生化與分子/生物信息學(xué)相關(guān)調(diào)劑 +5 | 星沉uu 2026-03-26 | 6/300 |
|
|
[考研] 環(huán)境專碩324分求調(diào)劑推薦 +5 | 軒小寧—— 2026-03-26 | 5/250 |
|
|
[考研] 【2026考研調(diào)劑】制藥工程 284分 求相關(guān)專業(yè)調(diào)劑名額 +4 | 袁奐奐 2026-03-25 | 8/400 |
|
|
[考研] 347求調(diào)劑 +4 | L when 2026-03-25 | 4/200 |
|
|
[考研] 285求調(diào)劑 +3 | AZMK 2026-03-24 | 3/150 |
|
|
[考研] 293求調(diào)劑 +7 | 加一一九 2026-03-24 | 7/350 |
|
|
[考研] 材料專碩331求調(diào)劑 +4 | 鮮當(dāng)牛 2026-03-24 | 4/200 |
|