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nnfyw木蟲 (著名寫手)
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[求助]
中譯英求助，375字
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| 用不同濃度的正己烷或二甲苯處理菌株OS1 和菌株UD2, 發(fā)酵后測定蛋白酶產(chǎn)量。結(jié)果如圖所示, 不同濃度有機(jī)溶劑脅迫處理均可以顯著提高兩株菌胞外蛋白酶產(chǎn)量。總的來講，隨著有機(jī)溶劑濃度的增加，蛋白酶分泌量增加，達(dá)到最大值后，增加有機(jī)溶劑濃度，蛋白酶分泌量不發(fā)生顯著變化。10%的正己烷和1%的二甲苯處理OS1以及12.5%的正己烷和5%的二甲苯處理UD2，蛋白酶分泌量達(dá)到最高。有趣的是有機(jī)溶劑脅迫處理不僅可以提高野生型菌株的蛋白酶產(chǎn)量, 也可以提高誘變育種獲得的突變菌株的蛋白酶產(chǎn)量。部分微生物通過增加胞蛋白酶分泌量提高其有機(jī)溶劑耐受性, 因此,有機(jī)溶劑脅迫會提高蛋白酶產(chǎn)量。研究者發(fā)現(xiàn)在發(fā)酵培養(yǎng)基中添加有機(jī)溶劑可以提高蛋白酶產(chǎn)量, 但有機(jī)溶劑可能會造成環(huán)境污染, 并且去除發(fā)酵液中的有機(jī)溶劑相對困難。用有機(jī)溶劑脅迫處理后發(fā)酵產(chǎn)蛋白酶, 不用在培養(yǎng)基中添加有機(jī)溶劑, 降低成本, 減少工藝, 綠色環(huán)保。 |
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jeangong
木蟲之王 (文學(xué)泰斗)
- 翻譯EPI: 8
- 應(yīng)助: 129 (高中生)
- 金幣: 68743.7
- 散金: 4
- 紅花: 54
- 帖子: 56257
- 在線: 813.8小時
- 蟲號: 222140
- 注冊: 2006-03-21
- 專業(yè): 無機(jī)材料化學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★
sltmac: 金幣-5, 違規(guī)存檔, 禁止軟件翻譯，初犯，扣5金幣 2012-04-24 14:22:13
sltmac: 金幣-5, 違規(guī)存檔, 禁止軟件翻譯，初犯，扣5金幣 2012-04-24 14:22:13
| Determination of protease production, fermentation strains with different concentrations of n-hexane or xylene treatment OS1 and strains UD2. Results shown, different concentrations of organic solvent stress can be significantly increased by two bacteria extracellular protease production. Generally speaking, with increasing organic solvent concentration, the increase in protease secretion reaches a maximum value, the increase in the organic solvent concentration, and protease secretion is not a significant change. 10% hexane and 1% xylene processing OS1 and 12.5% hexane and 5% of the xylene treatment UD2, the highest protease secretion. Interestingly, the organic solvent stress can not only enhance the protease production of the wild-type strain, can also increase the mutation breeding mutant protease production. Part of the micro-organisms by increasing the amount of extracellular protease secretion increased organic solvent tolerance, therefore, the stress of organic solvents to improve protease production. The researchers found that the organic solvent added to the fermentation medium can improve the production of protease, organic solvents may cause environmental pollution, and the relatively difficult to remove the organic solvent in the fermentation broth. After the fermentation of organic solvent stress protease, not in the culture medium of organic solvents, reduce costs, reduce process green environmental. |
3樓2012-04-24 14:14:11
zhoucb
金蟲 (職業(yè)作家)
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★
nnfyw: 金幣+1, 翻譯EPI+1, 能否不用軟件翻譯，這個明顯不達(dá)標(biāo)啊 2012-04-24 11:59:59
sltmac: 翻譯EPI-1, EPI發(fā)放錯誤 2012-04-24 14:22:29
nnfyw: 金幣+1, 翻譯EPI+1, 能否不用軟件翻譯，這個明顯不達(dá)標(biāo)啊 2012-04-24 11:59:59
sltmac: 翻譯EPI-1, EPI發(fā)放錯誤 2012-04-24 14:22:29
| In different concentrations are hexane or deal with OS1 and xylene strains UD2 strains after fermentation determination protease production. The results as shown in figure, different concentration organic solvent stress can greatly improve the treatments are two strains of bacteria exocellular protease production. In general, with the increasing of the concentration of organic solvent, protease secreted increased to maximum capacity, increase the concentration of organic solvent, protease secreted quantity does not have a significant change. 10% of hexane and 1% of xylene is OS1 processing and 12.5% of hexane and 5% of xylene is UD2 processing, protease secreted reached the highest amount. Interesting is organic solvent handle stress can not only improve the wild type strains protease yield, improve mutation breeding of the strains of protease mutations for production. Part of the microbial by increasing the protease secreted quantity and improve its organic solvent tolerance, therefore, organic solvent stress will improve the protease production. The researchers found that in the fermentation add organic solvent can improve the protease yield, but organic solvents may cause environmental pollution, and remove the fermented liquid organic solvent relatively difficult. Use organic solvent stress after processing ferment produce protease, need not in culture add organic solvent, lower the cost and reduce the process, green environmental protection. |
2樓2012-04-24 10:25:57
_▕若¨|相依
木蟲 (正式寫手)
- 翻譯EPI: 41
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- 蟲號: 1716996
- 注冊: 2012-03-26
- 性別: MM
- 專業(yè): 電化學(xué)分析
【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
nnfyw: 金幣+9, 翻譯EPI+1, ★有幫助, 有一些幫助，謝謝 2012-05-02 22:34:27
sltmac: 金幣+20 2012-05-14 08:25:48
nnfyw: 金幣+9, 翻譯EPI+1, ★有幫助, 有一些幫助，謝謝 2012-05-02 22:34:27
sltmac: 金幣+20 2012-05-14 08:25:48
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Deal with bacterial strain OS1 and UD2 with different concentration of n-hexane or xylene , measure the protease yield after fermentation. The results are shown in the figure, stress treatments with different concentration of organic solvent can all greatly improve exocellular protease yield of the two bacteria strains. In summary , the protease yield increased along with the concentration of organic solvent increasing ,but after up to the maximum, , protease yield did not change obviously when increased the concentration of organic solvent. When deal with bacterial strain OS1 with 10% of hexane and 1% of xylene and UD2 with 12.5% of hexane and 5% of xylene, protease yield reached the highest. The interesting thing is that handle stress of organic solvent can not only improve the protease yield of wild bacterial strains, but also improve the protease yield of mutated bacterial strains obained by mutagenesis breeding. Part of the microorganism secrete by increasing exocellula protease。 不專業(yè)，請指教，嘿嘿  |
4樓2012-04-24 20:20:27
_▕若¨|相依
木蟲 (正式寫手)
- 翻譯EPI: 41
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- 金幣: 1738.9
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- 蟲號: 1716996
- 注冊: 2012-03-26
- 性別: MM
- 專業(yè): 電化學(xué)分析
5樓2012-05-02 22:48:55
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