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s771042285鐵蟲 (初入文壇)
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[求助]
跪求，加急論文摘要翻譯
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| 本實(shí)驗(yàn)通過對四川各地土樣的采集，用于ε-多聚賴氨酸(ε-poly-lysine，ε-PL)產(chǎn)生菌種的篩選與鑒定。實(shí)驗(yàn)中首先對采集回來的泥土用SG液體培養(yǎng)基進(jìn)行富集培養(yǎng)48h，再根據(jù)ε-PL帶正電荷并能與帶正電荷的亞甲基藍(lán)排斥形成透明圈的性質(zhì)，通過加入亞甲基藍(lán)（0.02g/L）和重鉻酸鉀（0.05g/L，作為抑菌劑）的SG固體培養(yǎng)基對ε-PL產(chǎn)生菌進(jìn)行初篩。其次，根據(jù)ε-PL是一種生物堿，就可以通過改良碘化鉍試劑鉀（Dragendorff）這種生物堿的顯色劑能與DR試劑形成磚紅色沉淀和甲基橙的磺酸基陰離子能與聚賴氨酸的氨基陽離子能形成陽離子對，在pH3~10的范圍內(nèi)能形成磚紅色沉淀的原理，通過對發(fā)酵上清液進(jìn)行顯色復(fù)篩。然后，再通過對篩選出的ε-PL產(chǎn)生菌進(jìn)行生長特征、形態(tài)特征、生理生化鑒定初步鑒定篩選出的菌屬，再通過16SrDNA的分子生物學(xué)鑒定方法最終確定出篩選出的菌種種類。 |
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s771042285
鐵蟲 (初入文壇)
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2樓2012-05-29 12:29:11
sosako
金蟲 (著名寫手)
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
Mally89: 金幣+1, 感謝應(yīng)助！~ 2012-05-31 23:31:33
Mally89: 金幣+25, 翻譯EPI+1 2012-05-31 23:33:11
Mally89: 金幣+1, 感謝應(yīng)助！~ 2012-05-31 23:31:33
Mally89: 金幣+25, 翻譯EPI+1 2012-05-31 23:33:11
| The soil samples collectedaround Sichuan in this experiment were used to screen and identify the production of microbial strain for the ε-poly-L-lysine (ε-poly-lysine，ε-PL) . First，the collected soil sample were given enrichment culture for 48 hours with SG liquid medium .According to the ε-PL belt positive charge and the positively charged methylene blue exclusion nature to form a transparent circle,the production of microbial strain for the ε-PL was screened originally by adding the SG solid medium containing methylene blue (0.02g / L, ) and potassium dichromate (0.05g / L, as a bacteriostatic agent) for screening. Secondly, according to the alkaloid of the ε-PL , through this alkaloid color reagent of improved bismuth iodide potassium (Dragendorff) to form brick-red precipitate with DR reagent，and the a sulfonic acid of anionmethyl orange to form ion pairs with the amino cation of the ε-PL, with the brick-red precipitate in pH3 ~ 10, the color rescreening of the culture supernatant was carried out. Then, by the identification of the growth characteristics，morphological and physiological and biochemicalof for the preliminary identification and screening out the production of microbial strain for the ε-PL, and the molecular biology identification of 16SrDNA , the bacteria were screened out ultimately . |
3樓2012-05-30 00:23:57
duzhichao
木蟲 (小有名氣)
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- 蟲號: 1817367
- 注冊: 2012-05-15
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- 專業(yè): 基因組學(xué)
s771042285: 金幣+25, 翻譯EPI+1, ★★★★★最佳答案, 謝啦 2012-05-30 09:21:42
Mally89: 金幣-25, 翻譯EPI-1 2012-05-31 23:32:55
Mally89: 金幣-25, 翻譯EPI-1 2012-05-31 23:32:55
| This experiment Sichuan around the soil sample collection, screening and identification for the ε-poly-L-lysine (ε-poly-lysine or ε-PL) to produce strains. First collection back to the soil experiments with SG liquid medium enrichment culture 48h, according to the ε-PL belt positive charge and with the positively charged methylene blue exclusion nature to form a transparent circle, joining forces with methylene blue (0.02g / L, ) and potassium dichromate (0.05g / L, ε-PL producing strains as a bacteriostatic agent) SG solid medium for screening. Secondly, according to the ε-PL is an alkaloid, through improved color reagent bismuth iodide reagent potassium (Dragendorff) of this alkaloid and DR reagent to form a sulfonic acid anion, brick-red precipitate and methyl orange can cations on the cation can be formed with poly-L-lysine, amino, and pH3 ~ 10 within the principle of the brick-red precipitate can be formed by the color of the culture supernatant rescreening. Then, through the growth characteristics of screening out the ε-PL producing strains, morphological, physiological and biochemical identification, preliminary identification of the genus screened, and through the identification of molecular biology 16SrDNA method ultimately determine the kind of filter out bacteria.你在好好修改修改。嘿嘿。 |
4樓2012-05-30 00:44:52
s771042285
鐵蟲 (初入文壇)
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- 注冊: 2012-03-04
- 性別: MM
- 專業(yè): 生物大分子結(jié)構(gòu)與功能
5樓2012-05-30 09:24:45
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