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[求助]
懸賞50金幣!!求助!翻譯一段反相色譜與質(zhì)譜英文操作步驟
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非常急。。。屹p50金幣!!求助高手! Peptides were separated on a 20-cm column with a 75 µm inner diameter packed with Dr. Maisch GmbH reversed-phase material Reprosil-Pur 120 C18-AQ, 1.9 µm resin (SinoAmerican Proteomics LLC). Separation was performed using 220-min runs on a Waters NanoAcquity liquid chromatography with a binary buffer system of 2% acetonitrile in 0.1% formic acid (buffer A) and 99.9% acetonitrile in 0.1% formic acid (buffer B) interfaced to a LTQ-Orbitrap Velos (Thermo Fisher Scientific). The sample was loaded onto the trap column at a flow rate of 10 µl/min for 1 min and then separated at a flow rate of 300 nl per min by an initial linear gradient of buffer B from 6% to 26% up to 180 min followed by a 26% to 80% buffer B gradient for 40 min. The mass spectrometer was operated in data-dependent mode, with one full scan acquisition in the Orbitrap at 30,000 resolution (at 400 m/z) with an automatic gain control (AGC) target value of 10^6 ions. The 10 most intense ions with a charge >1 were selected for tandem data-dependent analysis using a hybrid method of alternating collision-induced dissociation and higher energy collision dissociation fragmentation techniques (CID and HCD, respectively)1. CID scans were collected at an AGC target value of 5,000 with a maximum injection time of 25 ms. HCD scans were collected at 7,500 resolution with an AGC target value of 30,000 and a maximum injection time of 150 ms. Dynamic exclusion was set to +/- 0.005 m/z for 60 seconds with an early expiration count of 1 and signal to noise of 2.[ 來(lái)自科研家族 生化掘金 ] |

銀蟲 (小有名氣)
| 我是做液相的 所以只能翻譯液相部分 質(zhì)譜部分翻譯不好 呵呵 如果翻譯有問(wèn)題敬請(qǐng)指正 |


新蟲 (小有名氣)
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我只是知道他在說(shuō)什么,可是翻譯就麻煩了, 因?yàn)楹芏辔乙仓皇怯糜⑽母静恢乐形脑趺疵?br /> Orbitrap是2005年Finnigan出的東西,很多地方和四極離子阱有點(diǎn)象,也是離子的捕集阱,只是原理有點(diǎn)不同,性能比FTICR差一些,比一般的質(zhì)譜好。 這東西進(jìn)中國(guó)本來(lái)就不多,中國(guó)的質(zhì)譜書籍都是上個(gè)世紀(jì)的東西了,很多名詞都無(wú)法真正跟上現(xiàn)在儀器的發(fā)展。 比如automatic gain control (AGC)叫自動(dòng)增益控制,但字面上跟本看不出。 給你個(gè)建議吧,找熱電公司的AE問(wèn)一下?赡芨鼫(zhǔn)確,上面其實(shí)寫的全是分析時(shí)的機(jī)器參數(shù)。包括分辨率,MS/MS的兩種掃描模式及捕集離子量控制參數(shù)等等。 看來(lái)也沒(méi)法再幫你了,抱歉。 |

新蟲 (小有名氣)
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http://www.thermo.com.cn/Product1150.html 這里你可以看一看,說(shuō)不定會(huì)有什么發(fā)現(xiàn)。涉及機(jī)器參數(shù)設(shè)置的信息太多,如果不是對(duì)質(zhì)譜很熟,可能完全不知道在講什么。 |

金蟲 (小有名氣)

新蟲 (正式寫手)
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