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| 本帖產(chǎn)生 1 個(gè) 翻譯EPI ，點(diǎn)擊這里進(jìn)行查看 | ||
[求助]
請(qǐng)幫忙翻譯一段生物醫(yī)藥方面的內(nèi)容，謝謝~
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EXPERIMENTAL EXAMPLE 1-1: Inhibition of Degranulation of Mast Cells - β-Hexosaminidase Assay The rat basophilic leukemia cell line RBL-2H3 was seeded at a density of 2x105 cells/well in MEM supplemented with 15% FBS and 100 units/ml penicillin and streptomycin and incubated for 24 hours at 37ºC in a 5% CO2 atmosphere. After removal of the medium, the cells were incubated for 4 hours in a fresh MEM medium containing 25 ng/ml anti-DNP IgE. Then, the cells were washed with PIPES buffer (25mM PIPES, 119 mM NaCl, 5 mM KCl, 1 mM CaCl2, 0.4 mM MgCl2, 40 mM NaOH, 5.6 mM glucose, 0.1% BSA) before pre-incubation for 10 min. Thereafter, the cells were treated for 20 min with 25, 50 and 100 μg/ml the samples of Example 1 and then for 20 min with 50 ng/ml DNP-BSA. The reaction was terminated at 4ºC and the supernatant was plated in an amount of 25 μL/well into 96-well plates and reacted with 25 μL of 1 mM P-nitrophenyl-acetyl-β-D-glucosaminide at 37ºC for 1 hour in each well. The reaction was terminated with 0.1 M citrate buffer before absorbance at 405 nm was read in an ELISA reader. |
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2樓2012-06-18 10:39:37
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
Mally89: 金幣+1, 感謝應(yīng)助，歡迎常來哈！ 2012-06-21 19:56:05
scswpiyly: 金幣+10, 翻譯EPI+1, ★★★很有幫助, thanks！ 2012-06-25 17:08:31
Mally89: 金幣+1, 感謝應(yīng)助，歡迎常來哈！ 2012-06-21 19:56:05
scswpiyly: 金幣+10, 翻譯EPI+1, ★★★很有幫助, thanks！ 2012-06-25 17:08:31
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