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| 5 | 1/1 | 返回列表 |
| 查看: 388 | 回復(fù): 2 | |||
| 本帖產(chǎn)生 1 個 翻譯EPI ，點擊這里進行查看 | |||
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[求助]
請幫忙翻譯一段生物醫(yī)藥方面的內(nèi)容，謝謝~
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EXPERIMENTAL EXAMPLE 1-1: Inhibition of Degranulation of Mast Cells - β-Hexosaminidase Assay The rat basophilic leukemia cell line RBL-2H3 was seeded at a density of 2x105 cells/well in MEM supplemented with 15% FBS and 100 units/ml penicillin and streptomycin and incubated for 24 hours at 37ºC in a 5% CO2 atmosphere. After removal of the medium, the cells were incubated for 4 hours in a fresh MEM medium containing 25 ng/ml anti-DNP IgE. Then, the cells were washed with PIPES buffer (25mM PIPES, 119 mM NaCl, 5 mM KCl, 1 mM CaCl2, 0.4 mM MgCl2, 40 mM NaOH, 5.6 mM glucose, 0.1% BSA) before pre-incubation for 10 min. Thereafter, the cells were treated for 20 min with 25, 50 and 100 μg/ml the samples of Example 1 and then for 20 min with 50 ng/ml DNP-BSA. The reaction was terminated at 4ºC and the supernatant was plated in an amount of 25 μL/well into 96-well plates and reacted with 25 μL of 1 mM P-nitrophenyl-acetyl-β-D-glucosaminide at 37ºC for 1 hour in each well. The reaction was terminated with 0.1 M citrate buffer before absorbance at 405 nm was read in an ELISA reader. |
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古可ぷ
榮譽版主 (文壇精英)
- 翻譯EPI: 323
- 應(yīng)助: 91 (初中生)
- 貴賓: 16.835
- 金幣: 19237.1
- 散金: 40372
- 紅花: 275
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- 專業(yè): 藥物設(shè)計與藥物信息
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【答案】應(yīng)助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
Mally89: 金幣+1, 感謝應(yīng)助，歡迎常來哈！ 2012-06-21 19:56:05
scswpiyly: 金幣+10, 翻譯EPI+1, ★★★很有幫助, thanks！ 2012-06-25 17:08:31
Mally89: 金幣+1, 感謝應(yīng)助，歡迎常來哈！ 2012-06-21 19:56:05
scswpiyly: 金幣+10, 翻譯EPI+1, ★★★很有幫助, thanks！ 2012-06-25 17:08:31
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實驗1-1：肥大細胞脫顆粒-β-氨基己糖苷酶的抑制試驗 大鼠嗜堿性粒細胞系RBL-2H3種植到濃度為 2x105 個細胞/孔、含有15% FBS 和100單位/ml 青霉素和鏈霉素的MEM培養(yǎng)基中，37度下、5%co2環(huán)境中，培養(yǎng)24小時。移去培養(yǎng)介質(zhì)后，細胞在含有25 ng/ml 抗體免疫球蛋白E的新MEM培養(yǎng)基中培養(yǎng)4小時。之后，在預(yù)培養(yǎng)前用 PIPES 緩沖液(25mg/ml PIPES, 119 mg/ml NaCl, 5 mg/ml KCl, 1 mg/ml CaCl2, 0.4 mg/ml MgCl2, 40 mg/ml NaOH, 5.6 mg/ml 葡萄糖, 0.1% 牛血清蛋白)洗滌細胞。隨后，細胞用 25, 50 以及 100 μg/ml的實驗1樣品處理20分鐘，再用50 ng/ml 抗體-牛血清蛋白處理20分鐘。反應(yīng)在4……后終止，上清液以25 μL/孔的量加入96-孔的培養(yǎng)皿中，每孔加入25 μL1mg/ml的對硝基苯酚-乙酰基-β-D-氨基葡萄糖苷與之在37……反應(yīng)一小時。在用ELISA測定儀測定405nm處的吸光度前，用0.1mol/l的檸檬酸緩沖液終止反應(yīng)。 有些亂碼的自己添加上去  |
3樓2012-06-21 16:47:49
2樓2012-06-18 10:39:37
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