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yanruo鐵桿木蟲(chóng) (正式寫(xiě)手)
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急求翻譯--生物、藥學(xué)專業(yè)
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請(qǐng)教各位英語(yǔ)高手幾段文字的翻譯，非常感謝！ “3.1 誘導(dǎo)細(xì)胞凋亡 凋亡是包括自噬、壞死、衰老等細(xì)胞死亡方式中的一種。 化合物1能夠使人類非小細(xì)胞肺癌細(xì)胞株（PLA-801）發(fā)生凋亡，凋亡方式是激活一種內(nèi)源性抑制酶，利用該內(nèi)源性抑制酶切割DNA 鏈，使DNA 降解成180～200 bp 的小片段，進(jìn)而使細(xì)胞體凋亡。Montririttigri 等研究表明，從Stephania venosa (Blume) 中提取的化合物1及其乙醇提取物均對(duì)卵巢癌細(xì)胞株（Skov3）有細(xì)胞毒性。通過(guò)MTT 法證明，阿樸菲類化合物以DNA 降解的方式使卵巢癌細(xì)胞凋亡。 3.2 減少或抑制癌細(xì)胞擴(kuò)散 通過(guò)檢測(cè)有絲分裂指數(shù)和細(xì)胞周期來(lái)分析細(xì)胞的擴(kuò)散情況。細(xì)胞周期的進(jìn)行依賴于細(xì)胞周期素的激活和細(xì)胞周期蛋白依賴性激酶（CDKs）。其功能是在G1 期啟動(dòng)S 期，并且使分裂進(jìn)入G2/M 期。從Stephania venosa 中提取的化合物1及其乙醇提取物除了能使卵巢癌細(xì)胞凋亡以外，它們對(duì)腫瘤細(xì)胞的擴(kuò)散也有明顯的抑制作用�；衔�2對(duì)惡性神經(jīng)膠質(zhì)瘤的作用機(jī)制就是抑制癌細(xì)胞擴(kuò)散。Daneli 等通過(guò)流式細(xì)胞術(shù)研究表明，化合物2處理惡性神經(jīng)膠質(zhì)瘤細(xì)胞株（U138-MG）24 h以后，G2/M 期細(xì)胞所占的百分比顯著增加，表明化合物2是通過(guò)將細(xì)胞周期阻斷在G2/M 期從而減少癌細(xì)胞擴(kuò)散， 達(dá)到抗癌的效果�；衔�3與腫瘤細(xì)胞株共培養(yǎng)，5 h 內(nèi)細(xì)胞株的細(xì)胞周期停滯于G2/M 期，之后則停滯于G1 期，而細(xì)胞停滯于S 期的細(xì)胞株則完全停止了DNA復(fù)制。 3.3 抑制DNA 拓?fù)洚悩?gòu)酶 DNA 拓?fù)洚悩?gòu)酶是存在于細(xì)胞核內(nèi)的一類酶，能夠催化DNA 鏈的斷裂和結(jié)合，從而控制DNA 的拓?fù)錉顟B(tài)。 生物堿獨(dú)特的母核結(jié)構(gòu)決定了該類化合物分子內(nèi)能呈現(xiàn)一個(gè)相對(duì)平面的結(jié)構(gòu)，使其能選擇性地與DNA 拓?fù)洚悩?gòu)酶II 的目標(biāo)DNA高效結(jié)合，形成的分子復(fù)合物很難解離，生物堿通過(guò)這種競(jìng)爭(zhēng)性結(jié)合抑制了DNA 拓?fù)洚悩?gòu)酶II 的催化活性而顯示出細(xì)胞毒性�；衔�4的抗癌機(jī)制就是它能鍵合插入到DNA 鏈中，通過(guò)與拓?fù)洚悩?gòu)酶競(jìng)爭(zhēng)奪取拓?fù)洚悩?gòu)酶的目標(biāo)DNA 而抑制其活性，從而達(dá)到抗癌作用。 ” [ Last edited by yanruo on 2012-7-22 at 22:06 ] |
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2樓2012-07-23 15:59:18
yanruo
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3樓2012-07-23 20:56:43
xn8008
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yanruo: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝您！ 2012-08-08 20:50:42
yanruo: 金幣+15, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝您！ 2012-08-08 20:50:42
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3.1Induction of apoptosis Apoptosis, including an autophagy, necrosis, senescence and cell death in one way. Compounds able to make a human non-small cell lung cancer cell lines (PLA-801) apoptosis undergo. Apoptosis is activating an endogenous inhibition of enzymes, the endogenous inhibitor enzymes cut the DNA strand, DNA is degradatied into small fragment between 180 ~ 200 bp, thereby apoptosis enable happened. Montririttigri and others from studies have shown that compounds extracted from Stephania venosa (Blume), an ethanol extract of ovarian cancer cell line (Skov3) cytotoxicity. A Park Philippine class of compounds to DNA degradation ovarian cancer cell lines by MTT assay proved. 3.2 Cancer cell proliferation is reduced or inhibited By detecting the mitotic index and cell cycle analysis of cell proliferation. The cell cycle dependent cyclin activation and cyclin-dependent kinase (of CDKs). Its function is to start in the G1 phase to S phase, and the split into the G2 / M phase. Compounds extracted from Stephania venosa and its ethanol extract addition to make ovarian cancer cell apoptosis, they proliferation of tumor cells are significantly inhibited. Compound 2 malignant gliomas, the mechanism of action is to inhibit cancer cell proliferation. Daneli with flow cytometry showed that the compound treatment of malignant glioma cell lines (U138-MG) for 24 h, the percentage of G2 / M phase cells increased significantly, indicating that the compound 2 by the cell cycle resistance off in the G2 / M phase to reduce the spread of cancer to achieve anti-cancer effect. Compound 3 with tumor cell lines co-cultured cell lines 5h of cell cycle arrest at G2 / M phase, and then arrest in G1 phase cell cycle arrest in S phase of the cell lines is the complete cessation of DNA replication. 3.3 Inhibition of DNA topoisomerase DNA topoisomerase is present in the nucleus of a class of enzymes capable of catalyzing DNA strand breaks and combined to control the state of DNA topology. Alkaloids unique nucleus structure determines the molecules of these compounds show a relatively flat structure, so that it selectively with DNA topoisomerase II, the target DNA efficient combination of the formation of molecular complexes is difficult to dissociation alkaloids through this competitive binding inhibition of DNA topoisomerase II catalytic activity and show cytotoxicity. The anticancer mechanism of compound 4 is that it co-inserted into the DNA strand through and topoisomerase competition to win the topoisomerase target DNA and inhibits its activity, so as to achieve the anti-cancer effect." |
4樓2012-07-30 09:48:03
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