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Biotljy木蟲 (小有名氣)
斗戰(zhàn)圣佛
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[求助]
關于擬南芥TCH基因響應機械刺激的相關課題討論
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| 請各位高手指點一下,在一般的實驗室條件下,如何實現(xiàn)擬南芥根的機械刺激,以及TCH基因及其編碼的鈣調蛋白及類似蛋白在響應其過程的作用!!急! |
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專家顧問 (職業(yè)作家)
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是這篇文章,方法如下 Arabidopsis roots were mechanically stimulated following three different protocols: (1) Individual epidermal cells were touched by rubbing a glass micropipette tip along the cell surface using a micromanipulator. (2) Roots were bent in the proximal elongation/mature zone. To impose a bend, the base of the root was immobilized in agarose, and the exposed part of the root was flexed with the aid of a glass capillary. (3) Vertically oriented roots immersed in agarose were grown into a barrier consisting of a piece of coverglass placed perpendicular to the root long axis Ca2+ Regulates Reactive Oxygen Species Production and pH during Mechanosensing in Arabidopsis Roots Gabriele B. Monshausen, Tatiana N. Bibikova, Manfred H. Weisenseel and Simon Gilroy The Plant Cell August 2009 vol. 21 no. 8 2341-2356 |

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