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Biotljy木蟲 (小有名氣)
斗戰(zhàn)圣佛
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[求助]
關(guān)于擬南芥TCH基因響應(yīng)機(jī)械刺激的相關(guān)課題討論
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| 請(qǐng)各位高手指點(diǎn)一下,在一般的實(shí)驗(yàn)室條件下,如何實(shí)現(xiàn)擬南芥根的機(jī)械刺激,以及TCH基因及其編碼的鈣調(diào)蛋白及類似蛋白在響應(yīng)其過(guò)程的作用。。〖! |
專家顧問(wèn) (職業(yè)作家)
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專家經(jīng)驗(yàn): +426 |
| 以前看過(guò)文章,研究機(jī)械刺激對(duì)根部細(xì)胞內(nèi)部信號(hào)影響,直接用玻璃探針接觸。目前對(duì)TCH產(chǎn)物功能好像還沒什么進(jìn)展,只能確認(rèn)他們是一系列鈣調(diào)蛋白,機(jī)械刺激后,植物內(nèi)源鈣水平發(fā)生變化,這一鈣信號(hào)上調(diào)激活蛋白激酶,最終上調(diào)某些TCH表達(dá)(如TCH2,3,4,TCH1不單純由鈣信號(hào)激活),同時(shí)有報(bào)道蛋白磷酸酶對(duì)某些TCH(如TCH3負(fù)調(diào)控)。 |

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專家顧問(wèn) (職業(yè)作家)
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專家經(jīng)驗(yàn): +426 |
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是這篇文章,方法如下 Arabidopsis roots were mechanically stimulated following three different protocols: (1) Individual epidermal cells were touched by rubbing a glass micropipette tip along the cell surface using a micromanipulator. (2) Roots were bent in the proximal elongation/mature zone. To impose a bend, the base of the root was immobilized in agarose, and the exposed part of the root was flexed with the aid of a glass capillary. (3) Vertically oriented roots immersed in agarose were grown into a barrier consisting of a piece of coverglass placed perpendicular to the root long axis Ca2+ Regulates Reactive Oxygen Species Production and pH during Mechanosensing in Arabidopsis Roots Gabriele B. Monshausen, Tatiana N. Bibikova, Manfred H. Weisenseel and Simon Gilroy The Plant Cell August 2009 vol. 21 no. 8 2341-2356 |

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