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林下之夕銅蟲 (小有名氣)
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[求助]
液相菜鳥求助
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這幾天做液相拖尾因子相當(dāng)嚴(yán)重 怎么處理啊 那個(gè)進(jìn)樣針新買的用了一次口上就有好多黑色氧化物 怎樣清洗與維護(hù) |
銅蟲 (初入文壇)

新蟲 (初入文壇)
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液相拖尾因子相當(dāng)嚴(yán)重 1. Check you concentration. See if you overload your sample. 2. Check if other impurity peaks hide in your main peak. 3. Change other columns to see any help. 4. Search literature and understand your compound's chemistry and find a better Mobile phase. 那個(gè)進(jìn)樣針新買的用了一次口上就有好多黑色氧化物 怎樣清洗與維護(hù) 1.Check your MP. What is your pH for MP. It should be higher than 2. Don't use HCl to adjust pH. 2. Post wash your needle for every injection. After run the whole sequence, set up a wash method with a long time water wash. |
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