| 24小時(shí)熱門(mén)版塊排行榜 |
| 5 | 1/1 | 返回列表 |
| 查看: 973 | 回復(fù): 10 | ||||
| 當(dāng)前只顯示滿足指定條件的回帖,點(diǎn)擊這里查看本話題的所有回帖 | ||||
juan_819鐵蟲(chóng) (初入文壇)
|
[求助]
如何提取HMGR
|
|||
|
請(qǐng)問(wèn): 如何從植物中提取HMGR這個(gè)酶從而用以進(jìn)行酶活測(cè)定? 謝謝! |

專家顧問(wèn) (職業(yè)作家)
![]() |
專家經(jīng)驗(yàn): +426 |

鐵蟲(chóng) (初入文壇)
專家顧問(wèn) (職業(yè)作家)
![]() |
專家經(jīng)驗(yàn): +426 |
|
以前我是照這個(gè)方法做得 All enzymes assayed in this work were extracted from the hairy roots with an extraction buffer of 50 mM Tris–HCl, 10 mM β-mercaptoethanol, 1% (w/v) polyvinylpyrrolidone and pH7.5. The roots were ground in the extraction buffer (1 g FW/mL) for 5 min with a pestle and mortar on ice, followed by centrifugation at 14,000 rpm and 4°C for 30 min to obtain a solids-free extract. The protein content was determined by the Bradford method using bovine serum albumin as a standard. |

| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 304求調(diào)劑 +8 | 素年祭語(yǔ) 2026-03-31 | 11/550 |
|
|---|---|---|---|---|
|
[考研] 311求調(diào)劑 +7 | 李芷新1 2026-03-31 | 7/350 |
|
|
[考研] 085600,321分求調(diào)劑 +7 | 大饞小子 2026-03-31 | 7/350 |
|
|
[考研] 328求調(diào)劑 +5 | 鵬鵬碰嘭嘭 2026-03-24 | 5/250 |
|
|
[考研] 329求調(diào)劑,一志愿西北工業(yè)大學(xué),材料工程(085601) +6 | 小小機(jī)靈蟲(chóng) 2026-03-29 | 12/600 |
|
|
[考研] 材料專碩調(diào)劑 +13 | 椰椰。 2026-03-29 | 13/650 |
|
|
[考研] 材料與化工(0856)304求B區(qū)調(diào)劑 +6 | 邱gl 2026-03-30 | 11/550 |
|
|
[考研] 334分 一志愿武理-080500 材料求調(diào)劑 +9 | 李李不服輸 2026-03-25 | 9/450 |
|
|
[考研] 一志愿西交大080500材料學(xué)碩349 +5 | jqx1258 2026-03-31 | 6/300 |
|
|
[基金申請(qǐng)] 面上5B能上會(huì)嗎? +8 | redcom 2026-03-29 | 8/400 |
|
|
[考研] 福建理工大學(xué)材料學(xué)院先進(jìn)合金團(tuán)隊(duì)招收考研調(diào)劑學(xué)生 +3 | 大華金商都 2026-03-30 | 4/200 |
|
|
[考研] 285求調(diào)劑 +6 | AZMK 2026-03-29 | 9/450 |
|
|
[考研] 289求調(diào)劑 +16 | 新時(shí)代材料 2026-03-27 | 16/800 |
|
|
[考研] 26考研-291分-廈門(mén)大學(xué)(085601)-柔性電子學(xué)院材料工程專業(yè)求調(diào)劑 +5 | min3 2026-03-24 | 6/300 |
|
|
[考研] 求調(diào)劑 +10 | 家佳佳佳佳佳 2026-03-29 | 10/500 |
|
|
[考研] 293求調(diào)劑 +3 | 末未mm 2026-03-30 | 5/250 |
|
|
[考研] 328求調(diào)劑 +8 | 嗯滴的基本都 2026-03-27 | 8/400 |
|
|
[考研] 348求調(diào)劑 +6 | 小懶蟲(chóng)不懶了 2026-03-28 | 6/300 |
|
|
[考研] 打過(guò)很多競(jìng)賽,085406控制工程300分,求調(diào)劑 +3 | askeladz 2026-03-26 | 3/150 |
|
|
[考研] 各位老師您好:本人初試372分 +5 | jj涌77 2026-03-25 | 6/300 |
|