24小時(shí)熱門版塊排行榜

返回列表

summer2771

木蟲 (正式寫手)

應(yīng)助: 1 (幼兒園)
金幣: 12256.9
散金: 118
紅花: 4
帖子: 875
在線: 235.9小時(shí)
蟲號(hào): 680481
注冊(cè): 2008-12-23
專業(yè): 細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)

[求助] 做信號(hào)通路的，是不是要做定位才好��？

請(qǐng)問(wèn)大家，大家一般做通路蛋白，都做了定位嘛？比如免疫熒光�。恐x謝！我看很多文章也都只做了蛋白表達(dá)，沒有做定位的。但是熒光圖確實(shí)漂亮，感覺能出彩些。不知道通路蛋白是不是這樣的？

回復(fù)此樓

» 猜你喜歡

食品專碩一志愿雙一流 328 已經(jīng)有4人回復(fù)
材料調(diào)劑已經(jīng)有6人回復(fù)
300分，材料，求調(diào)劑，英一數(shù)二已經(jīng)有5人回復(fù)
求調(diào)劑，一志愿:南京航空航天大學(xué)大學(xué) ，080500材料科學(xué)與工程學(xué)碩，總分289分已經(jīng)有5人回復(fù)
085600材料與化工調(diào)劑已經(jīng)有13人回復(fù)
材料與化工考研調(diào)劑已經(jīng)有7人回復(fù)
0856材料專碩353求調(diào)劑已經(jīng)有6人回復(fù)
07化學(xué)280分求調(diào)劑已經(jīng)有6人回復(fù)
086003食品工程求調(diào)劑已經(jīng)有5人回復(fù)
08工學(xué)調(diào)劑已經(jīng)有16人回復(fù)

» 本主題相關(guān)價(jià)值貼推薦，對(duì)您同樣有幫助:

請(qǐng)問(wèn)做細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)通路的大神，能否只做磷酸化不做總量？已經(jīng)有7人回復(fù)
細(xì)胞信號(hào)通路大全已經(jīng)有493人回復(fù)
細(xì)胞信號(hào)通路研究初學(xué)者經(jīng)驗(yàn)交流已經(jīng)有23人回復(fù)
求與細(xì)胞炎癥相關(guān)的信號(hào)通路？已經(jīng)有8人回復(fù)
信號(hào)通路已經(jīng)有10人回復(fù)
研究細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)通路，建立細(xì)胞系，是否一定要敲出細(xì)胞內(nèi)源性蛋白分子？已經(jīng)有8人回復(fù)
最新細(xì)胞各種信號(hào)通路概括~cell上的~ 已經(jīng)有61人回復(fù)
【求助/交流】抗癌藥物-凋亡-western 已經(jīng)有17人回復(fù)
【求助/交流】關(guān)于蛋白影響信號(hào)通路已經(jīng)有4人回復(fù)
【求助/交流】信號(hào)通路如何研究？已經(jīng)有13人回復(fù)

1樓 2012-11-30 17:03:39

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

laoboshihou

金蟲 (正式寫手)

應(yīng)助: 33 (小學(xué)生)
金幣: 724.2
紅花: 2
帖子: 528
在線: 75.5小時(shí)
蟲號(hào): 1947736
注冊(cè): 2012-08-20
專業(yè): 神經(jīng)生物學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
summer2771: 金幣+8, 謝謝您的回帖！ 2012-12-01 01:05:17
summer2771: 金幣+8, ★★★★★最佳答案 2012-12-23 02:50:53

通路蛋白通常只做定量，但也有少數(shù)做定位的文章，樓主不妨一試。順便提供一篇范文供樓主參考。

FASEB J. 2009 Aug;23(8):2576-86. Epub 2009 Mar 19.
Cell-type specific activation of p38 and ERK mediates calcitonin gene-related peptide involvement in tolerance to morphine-induced analgesia.
Wang Z, Ma W, Chabot JG, Quirion R.
SourceDouglas Mental Health University Institute, 6875 Blvd. LaSalle, McGill University, Montreal, Quebec, Canada H4H 1R3.

Abstract
Tolerance to morphine-induced analgesia is a well-established phenomenon, often limiting its usefulness in the long-term treatment of pain. The mechanisms underlying tolerance are not well understood. We previously suggested a possible role for spinal calcitonin gene-related peptide (CGRP) in the development of tolerance to morphine-induced analgesia. In the present study, we demonstrate that CGRP is involved in morphine tolerance by differentially regulating the ERK-dependent up-regulation of IL-1beta, TNF-alpha, and microsomal prostaglandin E synthase-1 (mPGES-1) in astrocytes and p38-dependent up-regulation of IL-6 in microglia in the rat spinal cord. A 7-d treatment with morphine induced tolerance to the antinociceptive effect and increased phosphorylated ERK localized in astrocytes and phosphorylated p38 enriched in microglia, both effects being inhibited by blocking CGRP receptors. Interestingly, the inhibition of the ERK pathway suppressed the development of tolerance and morphine-induced up-regulation of IL-1beta, TNF-alpha, and mPGES-1. Blockade of p38 activity also inhibited the development of tolerance and morphine-induced IL-6 up-regulation. Taken together, these data suggest that chronic morphine induces the synthesis of CGRP, which in turn acts on CGRP receptors located on astrocytes and microglia to stimulate ERK and p38, respectively, leading to increased synthesis and release of proinflammatory mediators resulting in tolerance to morphine-induced analgesia.

贊一下(1人)

回復(fù)此樓

2樓2012-11-30 17:12:26

已閱回復(fù)此樓關(guān)注TA 給TA發(fā)消息送TA紅花 TA的回帖

henryxue

木蟲 (小有名氣)

BioEPI: 1
應(yīng)助: 19 (小學(xué)生)
金幣: 6043.7
紅花: 1
帖子: 92
在線: 76.1小時(shí)
蟲號(hào): 2009319
注冊(cè): 2012-09-18
專業(yè): 免疫生物學(xué)

【答案】應(yīng)助回帖

★ ★ ★ ★
感謝參與，應(yīng)助指數(shù) +1
summer2771: 金幣+4, ★有幫助, 謝謝您的回帖！說(shuō)的也很詳細(xì)！ 2012-12-01 01:05:33

免疫熒光并不是不可以作為信號(hào)通路說(shuō)明的方法，但是需要看合不合適。比如你的蛋白會(huì)在特定刺激下在胞內(nèi)聚集、定位到特殊的細(xì)胞器、有入核出核的變化，免疫熒光是非常直觀的說(shuō)明手段，但是對(duì)于胞漿均勻分布的蛋白、對(duì)于銜接蛋白等等，這些用免疫熒光來(lái)說(shuō)明就并不合適。另外，免疫熒光的抗體質(zhì)量也是決定最后圖片漂亮與否的關(guān)鍵因素之一。最后廢話一句，實(shí)驗(yàn)室里有什么樣的試劑、設(shè)備以及可行的方法和技術(shù)，才是決定你實(shí)驗(yàn)設(shè)計(jì)的關(guān)鍵，如果沒有好用的抗體，熒光顯微鏡也不給力，做免疫熒光反而會(huì)畫蛇添足。

贊一下(3人)

回復(fù)此樓