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竹影靜亭新蟲 (初入文壇)
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[求助]
引物設(shè)計
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| 我要做染料法熒光定量,但是我用Primer設(shè)計,退貨溫度都很低,得分高的退貨溫度只有49度,只有一個溫度上50的,但是與模版有錯配,這應(yīng)該怎么弄啊,剛學(xué)設(shè)計引物,請各位給予知道!先將基因序列上傳, |
新蟲 (初入文壇)
鐵桿木蟲 (職業(yè)作家)
鐵桿木蟲 (職業(yè)作家)
至尊木蟲 (文壇精英)
巫山云
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December 23, 2012 Produced by Primer Premier, PREMIER Biosoft International, Tel: 650.856.2703, Fax: 650.843.1250, www.PremierBiosoft.com Primer Analysis Report Page: 1 Sequence: NewSequence (Sense primer) 5' CGCTGTTCTCCTTGCTTA 3' 3' (143) GCGACAAGAGGAACGAAT (160) 5' (Anti-sense primer) 5' GTCGTCCGTGATGGGTAT 3' 3' (724) CAGCAGGCACTACCCATA (707) 5' Properties: Rating 100 100 100 Seq No 143 724 -- Length 18 18 582 Tm [ ° C] 51.8 52.7 87.5 GC% 50.0 55.6 44.8 G [kcals/mol] -34.5 -34.3 -- Activity [ g/OD] 36.2 32.6 -- Degeneracy 1 1 -- Ta Opt [ ° C] -- -- 51.8 Sense Anti-sense Product Secondary structures of sense primer: No hairpins found No dimers found No false priming sites found Secondary structures of anti-sense primer: No hairpins found No dimers found No false priming sites found Secondary structures of primer pair: No Cross Dimers Found |

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