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yind1014鐵蟲 (初入文壇)
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| A reversed-phased HPLC method that allows the separation and simultaneous determination of the preservatives benzoic (BA) and sorbic acids (SA), methyl- (MP) and propylparabens (PP) is described. The separations were effected by using an initial mobile phase of methanol–acetate buffer (pH 4.4) (35:65) to elute BA, SA and MP and changing the mobile phase composition to methanol–acetate buffer (pH4.4) (50:50) thereafter. The detector wavelength was set at 254 nm. Under these conditions, separation of the four components was achieved in less than 23 min. Analytical characteristics of the separation such as limit of detection, limit of quantification, linear range and reproducibility were evaluated. The developed method was applied to the determination of 67 foodstuffs (mainly imported), comprising soft drinks, jams, sauces, canned fruits/vegetables, dried vegetables/fruits and others. The range of preservatives found were from not detected (nd)—1260,nd—1390, nd—44.8 and nd—221 mg kg−1for BA, SA, MP and PP, respectively. |
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Carena: 金幣+2, 感謝應(yīng)助 2012-12-21 13:53:45
yind1014: 金幣+5, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 跪謝�。。� 2012-12-21 20:37:07
Carena: 金幣+2, 感謝應(yīng)助 2012-12-21 13:53:45
yind1014: 金幣+5, 翻譯EPI+1, ★★★★★最佳答案, 非常感謝 跪謝�。。� 2012-12-21 20:37:07
| 用于對防腐劑苯甲酸（BA）和山梨酸（SA），尼泊金甲酯（MP），和對羥基苯甲酸丙酯（PP）進行分離和同時測定的反相高效液相色譜的方法被發(fā)現(xiàn)。通過用甲醇-乙酸鹽緩沖液（pH值4.4）（35:65）作為流動相進行洗脫來實現(xiàn)BA的分離，對于SA和MP的分離則通過將流動相改變?yōu)榧状?醋酸緩沖液（pH4.4）（50： 50）來實現(xiàn)。檢測波長設(shè)置為254nm。在這些條件下，四種化合物在23min內(nèi)成功的分離。本文對如檢測線、定量限、線性范圍和重現(xiàn)性等分析參數(shù)做出了評價。所建立的方法適用于測定67種食品（主要是進口的），包括汽水，果醬，調(diào)味料，水果/蔬菜罐頭，干蔬菜/水果等。對于BA、SA、MP和PP等防腐劑的檢測的范圍分別是1260ng、1390ng、44.8ng和221mg/kg。 |
2樓2012-12-21 09:10:58
yind1014
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3樓2012-12-21 20:37:33
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