 |
 |
 |
 |
 |
 |
||
 |
 |
 |
 |
 |
| 4 | 1/1 | 返回列表 |
| 查看: 2180 | 回復(fù): 3 | ||
[求助]
有沒有人用過去DNA甲基化的酶？
|
| 有沒有可以在體外進行DNA脫甲基化的酶？有人用過么？ |
回復(fù)此樓» 猜你喜歡
不合理蛙科研實驗中的趣事：實驗器材的 “烏龍”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗中的趣事：和實驗材料的 “斗智斗勇”
已經(jīng)有0人回復(fù)
-
化學(xué)工程及工業(yè)化學(xué)論文潤色/翻譯怎么收費?
已經(jīng)有238人回復(fù)
-
蛋白質(zhì)檢測：精準分析，解鎖生物分子的密碼
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之小鼠實驗：嚴謹設(shè)計，解析生命機制的重要載體
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之重金屬檢測：精準篩查，守護健康與環(huán)境的防線
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之“蛙測重金屬我背鍋三千”
已經(jīng)有0人回復(fù)
-
不合理蛙科研實驗之“鼠逃三次我發(fā)三篇SCI”
已經(jīng)有0人回復(fù)
-
納米粒度分析
已經(jīng)有3人回復(fù)
-
林科院林化所招收材料與化工專業(yè)碩士，坐標南京
已經(jīng)有0人回復(fù)
» 本主題相關(guān)價值貼推薦，對您同樣有幫助:
- 請問提出rna來還有三條帶，為什么dna酶消化檢測后后，就拖帶了？
已經(jīng)有10人回復(fù)
- 反轉(zhuǎn)錄前 DNA 酶消化
已經(jīng)有10人回復(fù)
- 質(zhì)粒酶切切不動，質(zhì)粒在宿主中繁衍酶切位點會突變么？
已經(jīng)有8人回復(fù)
- 求助MSP凝膠電泳該如何選擇
已經(jīng)有16人回復(fù)
- DNA甲基化檢測技術(shù)
已經(jīng)有10人回復(fù)
- 大家有沒有做過DNA甲基化的實驗?
已經(jīng)有9人回復(fù)
- 使用亞硫酸氫鈉測序法測試基因CpG甲基化
已經(jīng)有15人回復(fù)
- 有沒有人做太湖底泥微生物DNA提取的啊
已經(jīng)有8人回復(fù)
- 【求助/交流】定點突變PCR后加DPN1酶的作用
已經(jīng)有4人回復(fù)
- 有關(guān)感受態(tài)細胞DH5a的基因型
已經(jīng)有6人回復(fù)
ADTechnology 
捐助貴賓 (著名寫手)
表觀遺傳學(xué)專業(yè)供應(yīng)商
- 應(yīng)助: 12 (小學(xué)生)
- 金幣: 12092.7
- 散金: 1762
- 紅花: 1
- 帖子: 2608
- 在線: 49.1小時
- 蟲號: 1787705
- 注冊: 2012-04-30
- 專業(yè): 人類遺傳學(xué)
【答案】應(yīng)助回帖
★ ★ ★ ★ ★
liuxiuaza: 金幣+5 2013-06-04 12:52:32
liuxiuaza: 金幣+5 2013-06-04 12:52:32
|
超純DNA去甲基化活性/抑制分析試劑盒 貨號：A-P-3008 EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit (48 assays) 背景資料：DNA demethylation is necessary for the epigenentic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression. Demethylation of DNA can either be passive or active, or a combination of both. Passive DNA demethylation usually takes place on newly synthesized DNA strands via DNMT1 during replication rounds. Active DNA demethylation mainly occurs by the removal of 5-methylcytosine through further modified cytosine bases which have been converted by TET enzyme-mediated oxidation. These oxidation products have been shown to be repaired by TDG, a glycosylase which is involved in base excision repair, or directly converted to cytosine by DNMT3A/ DNMT3B in oxidative states. It is proposed that DNA demethylation could also be initiated by deamination of 5-mC through candidate deaminases including AID and APOBEC1, which convert 5-mC to thymine. The resulting thymine could be repaired by BER initiated by a T-G mismatch glycosylase such as MBD4 or TDG. In addition, the 5-mC base can be directly removed in plants by the DME/ROS1 family of 5-mC DNA glycosylases, resulting in an abasic site that is repaired by the BER process. 產(chǎn)品描述：The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Ultra Kit is a complete set of essential components which enables an experimenter to measure total DNA demethylase activity/inhibition. The kit can be used with nuclear extracts from a broad range of species such as mammals and plants, in a variety of forms including, but not limited to, cultured cells and fresh tissues. 產(chǎn)品特點： 1·Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 4 hours； 2·Safe and innovative colorimetric assay without the need for radioactivity, extraction, or chromatography； 3·An ultra-sensitive detection limit, as low as 1 µg of nuclear extract, which is five times more sensitive than the predecessor kit； 4·96 strip-well microplate format allows for either low or high throughput analysis。 不知道您是具體想做什么實驗?zāi)兀?img src="https://muchongimg.xmcimg.com/data/emuch_bbs_images/smilies/hand.gif" align="absmiddle" border="0"> |
2樓2013-06-01 17:07:16
lishiningdxp
新蟲 (初入文壇)
- 應(yīng)助: 0 (幼兒園)
- 金幣: 5.3
- 帖子: 7
- 在線: 9小時
- 蟲號: 4285488
- 注冊: 2015-12-13
- 專業(yè): 細胞、亞細胞結(jié)構(gòu)與功能
3樓2018-04-28 16:30:44
紛飛的葉
木蟲 (正式寫手)
- 應(yīng)助: 1 (幼兒園)
- 金幣: 5832.6
- 散金: 320
- 帖子: 559
- 在線: 52.9小時
- 蟲號: 2552847
- 注冊: 2013-07-18
- 專業(yè): 細胞生物學(xué)
4樓2018-04-29 09:26:45
相關(guān)版塊跳轉(zhuǎn)
我要訂閱樓主 liuxiuaza 的主題更新
| 4 | 1/1 | 返回列表 |
| 最具人氣熱帖推薦 [查看全部] | 作者 | 回/看 | 最后發(fā)表 | |
|---|---|---|---|---|
|
[考研] 一志愿985，本科211，0817化學(xué)工程與技術(shù)319求調(diào)劑 +7 | Liwangman 2026-03-15 | 7/350 |
|
|---|---|---|---|---|
|
|
[考研] 266求調(diào)劑 +5 | 陽陽哇塞 2026-03-14 | 9/450 |
|
|
[考研] 材料專碩306英一數(shù)二 +10 | z1z2z3879 2026-03-16 | 13/650 |
|
|
|
[考研] 0703化學(xué)調(diào)劑 +4 | pupcoco 2026-03-17 | 7/350 |
|
|
|
[考研] 278求調(diào)劑 +5 | 煙火先于春 2026-03-17 | 5/250 |
|
|
|
[考研] 材料與化工求調(diào)劑 +6 | 為學(xué)666 2026-03-16 | 6/300 |
|
|
|
[考研] 材料與化工專碩調(diào)劑 +5 | heming3743 2026-03-16 | 5/250 |
|
|
|
[考研]
 11408 一志愿西電，277分求調(diào)劑
+3
|
zhouzhen654 2026-03-16 | 3/150 |
|
|
|
[基金申請]
今年的國基金是打分制嗎？
 50+3
|
zhanghaozhu 2026-03-14 | 3/150 |
|
|
|
[考研] 0856求調(diào)劑 +3 | 劉夢微 2026-03-15 | 3/150 |
|
|
|
[考研] 326求調(diào)劑 +3 | mlpqaz03 2026-03-15 | 3/150 |
|
|
|
[考博] 東華理工大學(xué)化材專業(yè)26屆碩士博士申請 +6 | zlingli 2026-03-13 | 6/300 |
|
|
|
[考研] 294求調(diào)劑 +3 | Zys010410@ 2026-03-13 | 4/200 |
|
|
|
[考研] 329求調(diào)劑 +3 | miaodesi 2026-03-12 | 4/200 |
|
|
|
[考研] 281求調(diào)劑 +9 | Koxui 2026-03-12 | 11/550 |
|
|
|
[考研] 311求調(diào)劑 +3 | 冬十三 2026-03-13 | 3/150 |
|
|
|
[考研] 材料專碩350 求調(diào)劑 +4 | 王金科 2026-03-12 | 4/200 |
|
|
|
[考研] 求調(diào)劑 +3 | 程雨杭 2026-03-12 | 3/150 |
|
|
|
[考研] 328化工專碩求調(diào)劑 +4 | 。，。，。，。i 2026-03-12 | 4/200 |
|
|
|
[考研] 085600材料與化工 309分請求調(diào)劑 +7 | dtdxzxx 2026-03-12 | 8/400 |
|